Imaging of RNA in bacteria with self-ligating quenched probes
Imaging of RNA in bacteria with self-ligating quenched probes
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DOI：
10.1021/ja026649g
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发表时间：
2002-08-21
影响因子：
15
通讯作者：
Kool, ET
中科院分区：
文献类型：
--
作者：
Sando, S;Kool, ET
We report on the application of a new class of oligonucleotide reporter probes, QUAL probes, that “light up” when a nucleophilic phosphorothioate probe binds adjacent to a dabsyl-quenched probe. These self-ligating DNA probes were used for sequence-specific detection of 16S rRNA inEscherichia colicells. Strong fluorescence was observed only when the phosphorothioate and quenched dabsyl probes bind side-by-side on a 16S rRNA target. The results demonstrate the use of QUAL probes to detect specific RNA sequences in bacterial cells without enzymes and without washing steps.
