Imaging mouse embryonic development.
Imaging mouse embryonic development.
复制标题
成像小鼠胚胎发育。
DOI：
10.1016/s0076-6879(10)76019-5
复制
发表时间：
2010
影响因子：
--
通讯作者：
Dickinson ME
中科院分区：
文献类型：
--
作者：
Udan RS;Dickinson ME
在过去的三十年中，已经优化了用于离体培养小鼠胚胎的方法，以在胚胎发生的整个关键阶段提高胚胎活力和生理学。结合转基因动物的生产和不同品种的荧光蛋白（FP）的发展取得的进展，延时成像在小鼠发育过程中的动态事件分析中变得越来越流行。将FP靶向特定的细胞类型或亚细胞区室使研究人员能够真实的研究活小鼠胚胎中的细胞增殖、凋亡、迁移和细胞形态变化。在这里，我们为小鼠胚胎发育早期阶段的延时成像提供了指导。
For the past three decades, methods for culturing mouse embryos ex vivo have been optimized in order to improve embryo viability and physiology throughout critical stages of embryogenesis. Combining advances made in the production of transgenic animals and in the development of different varieties of fluorescent proteins (FPs), time-lapse imaging is becoming more and more popular in the analysis of dynamic events during mouse development. Targeting FPs to specific cell types or subcellular compartments has enabled researchers to study cell proliferation, apoptosis, migration, and changes in cell morphology in living mouse embryos in real time. Here we provide a guide for time-lapse imaging of early stages of mouse embryo development.