Bioactive Peptides Isolated from Casein Phosphopeptides Enhance Calcium and Magnesium Uptake in Caco-2 Cell Monolayers
Bioactive Peptides Isolated from Casein Phosphopeptides Enhance Calcium and Magnesium Uptake in Caco-2 Cell Monolayers
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DOI：
10.1021/acs.jafc.6b05711
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发表时间：
2017-03-22
影响因子：
6.1
通讯作者：
Xiao, Hang
中科院分区：
文献类型：
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作者：
Cao, Yong;Miao, Jianyin;Xiao, Hang
酪蛋白磷酸肽（CPPs）结合和运输矿物质的能力已经被研究过。然而，对CPPs作用负责的单一生物活性肽尚未被确定。本研究旨在从CPPs中纯化钙结合肽，并测定其对Caco-2细胞单层钙镁摄取的影响。分离得到P1 ~ P5 5个单体肽，采用LC-MS/MS测定氨基酸序列。与不含cppp的对照组相比，5种单体肽对钙和镁的摄取均有显著的促进作用（P < 0.05）。有趣的是，当钙和镁与P5同时呈递时，Caco-2细胞单层中镁优先于钙。例如，在180 min时，镁和钙的转运量分别为78.4 +/- 0.95 mu g/井和2.56 +/- 0.64 mu g/井，表明P5引起的转运量相差30倍以上。这些结果为从酪蛋白中获得的磷酸肽的矿物运输活性提供了新的见解。
The ability of casein phosphopeptides (CPPs) to bind and transport minerals has been previously studied. However, the single bioactive peptides responsible for the effects of CPPs have not been identified. This study was to purify calcium-binding peptides from CPPs and to determine their effects on calcium and magnesium uptake by Caco-2 cell monolayers. Five monomer peptides designated P1 to P5 were isolated and the amino acid sequences were determined using LC-MS/MS. Compared with the CPP-free control, all five monomeric peptides exhibited significant enhancing effects on the uptake of calcium and magnesium (P < 0.05). Interestingly, when calcium and magnesium were presented simultaneously with P5, Magnesium was taken up with priority over calcium in the Caco-2 cell monolayers. For example, at 180 min, the amount of transferred magnesium and calcium was 78.4 +/- 0.95 mu g/well and 2.56 +/- 0.64 mu g/well, respectively, showing a more than 30-fold difference in the amount of transport caused by P5. These results provide novel insight into the mineral transport activity of phosphopeptides obtained from casein.