OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis
OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis
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DOI：
10.1007/s11103-005-5702-5
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Chung, YY
中科院分区：
文献类型：
--
作者：
Gothandam, KM;Kim, ES;Chung, YY
本文报道了一个新的水稻五肽重复序列(PPR)蛋白基因。PPR是一个由35个氨基酸串联而成的特征性重复基序，广泛存在于包括植物在内的多种生物系统中。序列分析表明，该基因全长2433个核苷酸，编码8个10个氨基酸，包括11个PPR基序。BLAST搜索结果表明，该基因与植物中的任何一个特征PPR基因都没有同源性。研究发现，OsPPR1基因的N-末端含有一个可能的叶绿体转运肽，提示该基因产物针对叶绿体。Southern杂交结果表明，OsPPR1是水稻基因组中一个基因家族的成员。表达分析和免疫印迹分析表明，OsPPR1主要在水稻叶片中积累。利用反义转基因策略抑制OsPPR1的表达，得到的转基因水稻表现出典型的叶绿素缺乏突变体的表型：白化和致死。显微镜细胞学观察表明，反义转基因植株叶绿体发育存在明显缺陷。综上所述，这些结果表明OsPPR1是一个水稻核基因，编码的PPR蛋白可能在叶绿体生物发生中发挥作用。这是有关水稻叶绿体生物发生所需的PPR蛋白的首次报道。
In this paper, we report a novel pentatricopeptide repeat (PPR) protein gene in rice. PPR, a characteristic repeat motif consisted of tandem 35 amino acids, has been found in various biological systems including plant. Sequence analysis revealed that the gene designated OsPPR1 consisted of an open reading frame of 2433 nucleotides encoding 8 10 amino acids that include 11 PPR motifs. Blast search result indicated that the gene did not align with any of the characterized PPR genes in plant. The OsPPR1 gene was found to contain a putative chloroplast transit peptide in the N-terminal region, suggesting that the gene product targets to the chloroplast. Southern blot hybridization indicated that the OsPPR1 is the member of a gene family within the rice genome. Expression analysis and immunoblot analysis suggested that the OsPPR1 was accumulated mainly in rice leaf. Antisense transgenic strategy was used to suppress the expression of OsPPR1 and the resulted transgenic rice showed the typical phenotypes of chlorophyll-deficient mutants; albinism and lethality. Cytological observation using microscopy revealed that the antisense transgenic plant contained a significant defect in the chloroplast development. Taken together, the results suggest that the OsPPR1 is a nuclear gene of rice, encoding the PPR protein that might play a role in the chloroplast biogenesis. This is the first report on the PPR protein required for the chloroplast biogenesis in rice.