Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa
Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa
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DOI：
10.1128/jb.178.14.4297-4300.1996
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发表时间：
1996-07-01
影响因子：
3.2
通讯作者：
Filloux, A
中科院分区：
文献类型：
--
作者：
Bleves, S;Lazdunski, A;Filloux, A
Xcp蛋白构成铜绿假单胞菌的分泌器。推导的氨基酸序列的rep基因，表达和亚细胞定位揭示了意想不到的功能。事实上，大多数Xcp蛋白存在于细胞质膜中，尽管rep突变导致外蛋白的周质积累，表明限制步骤是跨外膜的易位。为了理解机械功能及其组件之间的相互作用的机制，了解它们的膜组织是有价值的。我们报告的数据表明三个一般的分泌途径组件，XcpP，-Y，和-Z蛋白的N-在-C-出拓扑结构。
Xcp proteins constitute the secretory apparatus of Pseudomonas aeruginosa. Deduced amino acid sequence of rep genes, expression, and subcellular localization revealed unexpected features. Indeed, most Xcp proteins are found in the cytoplasmic membrane although rep mutations lead to periplasmic accumulation of exoproteins, indicating that the limiting step is translocation across the outer membrane. To understand the mechanism by which the machinery functions and the interactions between its components, it is valuable to know their membrane organization. We report data demonstrating the N-in-C-out topologies of three general secretion pathway components, the XcpP, -Y, and -Z proteins.