Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages
Suppression of inflammation by ethanol extract of Clausena lansium via modulation of TLR4/MYD88/TRAF6 signaling pathway in RAW 264.7 macrophages
复制标题
黄皮乙醇提取物通过调节 RAW 264.7 巨噬细胞中的 TLR4/MYD88/TRAF6 信号通路抑制炎症
DOI：
10.1177/2058739219841973
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发表时间：
2019-04-15
影响因子：
0.7
通讯作者：
Xiao, Shengjun
中科院分区：
文献类型：
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作者：
Huang, Guihong;Juan Li;Xiao, Shengjun
在东方传统医学中，黄皮的果实和根是治疗支气管炎的药物。据报道，黄皮提取物具有抗氧化、抗炎和抗癌活性。本研究旨在探讨黄皮乙醇提取物是否可以抑制脂多糖 (LPS) 诱导的 RAW 264.7 巨噬细胞炎症及其潜在机制。酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）分别检测肿瘤坏死因子α（TNF-α）蛋白的分泌和TNF-α mRNA的表达。 RT-PCR 和 Western blotting 分别用于测定 TLR4、MYD88 和 TRAF6 的 mRNA 和蛋白水平。黄皮提取物抑制 TNF-α 的表达和分泌，TNF-α 是由 RAW 264.7 细胞在 LPS 诱导后释放的。同时，黄皮提取物降低了 TLR4、MYD88 和 TRAF6 的表达。同时，MYD88抑制剂NBP2-29328可协同增强黄皮提取物的抑制效果。结果表明，黄皮乙醇提取物可减轻巨噬细胞介导的炎症，TLR4/MYD88/TRAF6 通路是其潜在靶点。
The fruit and root of Clausena lansium are remedy for bronchitis in oriental traditional medicine. Extracts from Clausena lansium are reported to have antioxidant, anti-inflammatory, and anticancer activities. This study is designed to investigate whether the ethanol extract of Clausena lansium (Lour.) Skeels could inhibit the lipopolysaccharides (LPS)-induced inflammatory in RAW 264.7 macrophages and the underlining mechanisms. Enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) are used to detect the secretion of tumor necrosis factor alpha (TNF-α) protein and expression of TNF-α mRNA, respectively. RT-PCR and Western blotting are used to determine the mRNA and protein levels of TLR4, MYD88, and TRAF6, respectively. The extract of Clausena lansium suppressed the expression and secretion of TNF-α, which is released by RAW 264.7 cells after LPS induction. Meanwhile, the expression of TLR4, MYD88, and TRAF6 are decreased by extracts from Clausena lansium. Meanwhile, NBP2-29328, an inhibitor of MYD88, synergistically enhances the inhibiting effect of extract from Clausena lansium. The results imply that ethanol extract from Clausena lansium attenuate macrophage-mediated inflammation and TLR4/MYD88/TRAF6 pathway is its potential target.