Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact.
Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact.
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研究组蛋白甲基转移酶自动甲基化及其功能影响的技术。
DOI：
10.1007/978-1-0716-2481-4_5
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Lee,Chul-Hwan
中科院分区：
文献类型：
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作者：
Popoca,Luis;Lee,Chul-Hwan
组蛋白甲基转移酶的催化活性不仅限于组蛋白，还包括非典型底物。越来越多的证据表明，组蛋白甲基转移酶自身甲基化，自甲基化已成为一种自我调节机制。在这里，我们介绍了实验程序，以确定组蛋白甲基转移酶的自甲基化位点，并探讨自甲基化的功能，在重建的生化系统和细胞环境。
The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.