The SCAR/WAVE complex is necessary for proper regulation of traction stresses during amoeboid motility.
The SCAR/WAVE complex is necessary for proper regulation of traction stresses during amoeboid motility.
复制标题
疤痕/波复合物对于在变形虫运动过程中适当调节牵引力是必要的。
DOI：
10.1091/mbc.e11-03-0278
复制
发表时间：
2011-11
影响因子：
3.3
通讯作者：
Firtel RA
中科院分区：
文献类型：
--
作者：
Bastounis E;Meili R;Alonso-Latorre B;del Álamo JC;Lasheras JC;Firtel RA
牵引力和f -肌动蛋白测量的结合表明，缺乏SCAR/WAVE复合物蛋白SCAR和PIR121的细胞表现出细胞运动周期和牵引力应力时空分布的改变，其大小与f -肌动蛋白水平相关。细胞迁移需要一个严格调控的、时空协调的潜在生化途径。细胞迁移的关键是SCAR/波介导的树突状f -肌动蛋白聚合在细胞的前沿。我们的目标是了解SCAR/WAVE复合物在变形虫迁移机制中的作用。为此，我们测量并比较了缺乏SCAR/WAVE复合物蛋白PIR121 （pirA−）和SCAR （scrA−）的盘形骨细胞与野生型细胞在平面弹性基质上迁移时所施加的牵引应力。我们发现，与野生型相比，两种突变菌株施加不同强度的牵引应力，这与它们的f -肌动蛋白水平相关。与先前的研究一致，我们发现野生型细胞通过重复运动周期迁移，其中细胞长度和细胞对其底物施加的应变能周期性变化。我们的分析还表明，scrA -细胞表现出一种改变的运动周期，具有更长的周期和更低的迁移速度，而pirA -细胞以随机的方式迁移，而不实现周期性循环。我们详细描述了各种细胞系的牵引-应激表型，为f -肌动蛋白聚合在调节细胞-基质相互作用和运动所需的应激中的作用提供了新的见解。
A combination of traction force and F-actin measurements shows that cells lacking either of the SCAR/WAVE complex proteins SCAR and PIR121 exhibit an altered cell motility cycle and spatiotemporal distribution of tractions stresses, which correlate in magnitude with F-actin levels. Cell migration requires a tightly regulated, spatiotemporal coordination of underlying biochemical pathways. Crucial to cell migration is SCAR/WAVE–mediated dendritic F-actin polymerization at the cell's leading edge. Our goal is to understand the role the SCAR/WAVE complex plays in the mechanics of amoeboid migration. To this aim, we measured and compared the traction stresses exerted by Dictyostelium cells lacking the SCAR/WAVE complex proteins PIR121 (pirA−) and SCAR (scrA−) with those of wild-type cells while they were migrating on flat, elastic substrates. We found that, compared to wild type, both mutant strains exert traction stresses of different strengths that correlate with their F-actin levels. In agreement with previous studies, we found that wild-type cells migrate by repeating a motility cycle in which the cell length and strain energy exerted by the cells on their substrate vary periodically. Our analysis also revealed that scrA− cells display an altered motility cycle with a longer period and a lower migration velocity, whereas pirA− cells migrate in a random manner without implementing a periodic cycle. We present detailed characterization of the traction-stress phenotypes of the various cell lines, providing new insights into the role of F-actin polymerization in regulating cell–substratum interactions and stresses required for motility.