Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus
Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus
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鸡脾脏褪黑素受体表达的发育变化
DOI：
10.1016/j.acthis.2015.05.002
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Guo, Qingyun;Dong, Yulan;Chen, Yaoxing
褪黑激素通过其受体在禽类脾脏的发育和免疫调节中起着重要作用，然而褪黑激素受体亚型在禽类脾脏发育过程中的表达变化尚不清楚。本研究旨在探讨鸡脾脏褪黑素受体亚型（Mel 1a、Mel 1b和Mel 1c）表达的分布规律和发育变化，以及血浆褪黑素浓度与褪黑素受体亚型表达的相关性。免疫组化结果显示，Mel 1a主要分布于红髓和毛细血管，Mel 1b主要分布于动脉周围淋巴鞘（帕尔斯）和脾小结，Mel 1c广泛分布于红髓、帕尔斯和脾小结。从P0到P21，脾脏Mel 1a、Mel 1b和Mel 1c的mRNA和蛋白表达均增加（P <0. 05），但P21后Mel 1a、Mel 1b和Mel 1c的表达略有增加（P > 0. 05）。P0 ~ P14时，Mel 1b和Mel 1c的mRNA水平比Mel 1a的mRNA水平升高更快。从出生后到出生后42天，血浆褪黑素浓度呈日龄依赖性增加（P < 0.05），出生后14天后呈线性增加（P < 0.05）。血浆中褪黑素水平与鸡脾脏中Melia（r=0.938，P = 0.005）、Mel 1b（r=0.912，P=0.011）和Mel 1c（r=0.906，P=0.012）蛋白表达量呈显著正相关。这些结果表明鸡脾脏中褪黑素受体亚型的表达存在年龄相关和区域特异性变化，这种特征性模式可能参与了鸡脾脏的发育和功能成熟。(C)2015 Elsevier GmbH. All rights reserved.
Melatonin plays an essential role in development and immunoregulation of the avian spleen through its receptors; however, the variations in the expression of the melatonin receptor subtypes in the developing avian spleen are still unclear. The objective of the present study was not only to investigate the distribution patterns and development changes of the expression of the melatonin receptor subtypes (Mel1a, Mel1b and Mel1c) in the chicken spleen but also to identify the correlation between the plasma melatonin concentration and the expression of the melatonin receptor subtypes. The immunohistochemical results indicated that Mel1a was mainly distributed in the red pulp and capillaries, Mel1b was predominantly distributed in the periarterial lymphatic sheath (PALS) and splenic nodule, and Mel1c was widely located in the red pulp, PALS and splenic nodule. From PO to P21, the mRNA and protein expressions of Mel1a, Mel1b and Mel1c in the spleen were increased (P < 0.05); however, a slight increase in the expression of the three melatonin receptor subtypes was observed after P21 (P > 0.05). Furthermore, the mRNA levels of Mel1b and Mel1c between P0 and P14 raised more quickly than Mel1a. The plasma melatonin concentration increased in an age-dependent manner in the chicken from PO to P42 (P < 0.05), and this increasing change was linear after P14 (P < 0.05). The melatonin level in the plasma is strongly correlated with the protein expressions of Melia (r=0.938, P = 0.005), Mel1b (r=0.912, P=0.011), and Mel1c (r=0.906, P=0.012) in the chicken spleen. These results suggest the existence of age-related and regionspecific changes in the expression of the melatonin receptor subtypes within the spleen of the chicken, and this characteristic pattern may be involved in the development and functional maturation of the avian spleen. (C) 2015 Elsevier GmbH. All rights reserved.