Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang).
Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang).
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辣椒（Capsicum annuum cv. Pukang）中病原体诱导的环加氧酶同源物表达。
DOI：
10.1093/jexbot/53.367.383
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发表时间：
2002
影响因子：
6.9
通讯作者：
D. Choi
中科院分区：
文献类型：
--
作者：
Young;S. Yi;Hyunggon Mang;Young Sam Seo;W. Kim;D. Choi
植物的过敏反应（HR）表现为病原体感染部位的快速和局部细胞死亡。为了更好地了解控制HR的分子和细胞防御机制，辣椒叶片（辣椒品种。普康）接种大豆脓疱病原菌野油菜黄单胞菌致病变种（Xanthomonascampestris pv.）甘氨酸8 ra.利用DD-PCR技术，从烟草中分离到一个与烟草病原体诱导的加氧酶（PIOX）序列相似的cDNA片段，该片段与动物环加氧酶（考克斯）具有同源性。随后，从病原菌接种的辣椒叶片cDNA文库中分离出编码考克斯同源物的全长cDNA克隆pCa-COX 1。推导的Ca-COX 1的氨基酸序列与烟草PIOX的同源性为85.8%，与哺乳动物COXs的同源性为21.7-23.7%。Ca-COX 1的表达在病原菌感染后4-12 h被显著诱导，而辣椒叶片上的HR细胞在接种后约15 h出现死亡。这些结果是一致的概念，脂质衍生的信号通路参与辣椒植物病原体感染的初始反应。
The hypersensitive reaction (HR) in plants is typified by a rapid and localized cell death at the site of pathogen infection. To understand better the molecular and cellular defence mechanism controlling HR, hot pepper leaves (Capsicum annuum cv. Pukang) were inoculated with the soybean pustule pathogen Xanthomonas campestris pv. glycine 8ra. By using the DD-PCR technique, a cDNA fragment was identified that exhibited a sequence similarity to the recently identified tobacco pathogen-induced oxygenase (PIOX) with homology to animal cyclo-oxygenase (COX). Subsequently, the full-length cDNA clone, pCa-COX1, encoding the COX homologue from the pathogen-inoculated hot pepper leaf cDNA library was isolated. The deduced amino acid sequence of Ca-COX1 shares 85.8% identity with tobacco PIOX and displays a significant degree of sequence identity (21.7-23.7%) with mammalian COXs. The expression of Ca-COX1 was markedly induced at 4-12 h after pathogen infection, while HR cell death on pepper leaves appeared at approximately 15 h post-inoculation. These results are consistent with the notion that the lipid-derived signalling pathway is involved in the initial response of hot pepper plants to pathogen infection.
DOI：
10.1073/pnas.85.5.1412
发表时间：
1988-03-01
影响因子：
11.1
作者：
DEWITT, DL;SMITH, WL
通讯作者：
SMITH, WL
DOI：
10.1073/pnas.89.11.4888
发表时间：
1992-06-01
影响因子：
11.1
作者：
OBANION, MK;WINN, VD;YOUNG, DA
通讯作者：
YOUNG, DA