Effect of gut microbiota on in vitro bioaccessibility of heavy metals and human health risk assessment from ingestion of contaminated soils
Effect of gut microbiota on in vitro bioaccessibility of heavy metals and human health risk assessment from ingestion of contaminated soils
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肠道微生物群对重金属体外生物可及性的影响以及摄入受污染土壤的人类健康风险评估
DOI：
10.1016/j.envpol.2021.116943
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发表时间：
2021
影响因子：
8.9
通讯作者：
Cui Yanshan
中科院分区：
文献类型：
--
作者：
Yin Naiyi;Zhao Yongli;Wang Pengfei;Du Huili;Yang Mei;Han Zeliang;Chen Xiaochen;Sun Guoxin;Cui Yanshan
为明确肠道菌群在人体健康风险评价中的作用，采用模拟胃肠液法测定了14个土壤样品中重金属的生物可利用性。与小肠相相比，结肠相的生物可及性值各不相同，As增加3.5倍，Cr增加2.2倍，Ni增加1.6倍，Cu减少4.4倍。在大多数土壤样品中，成人肠道菌群的结肠孵育产生了比儿童更高的As（1.3倍）和Fe（3.4倍）的生物可利用性值。结肠生物可利用性是约60%以上的镉为成人和30%以上的铬为儿童。观察到关于Cu和Ni的生物可及性的一致数据。相关分析表明，土壤重金属的生物可利用性主要与土壤重金属总量有关，其次是土壤pH值和活性铁锰氧化物。值得注意的是，基于结肠生物可及性计算的风险评估表明，成人的3个土壤样本（1.05-3.35）和儿童的9个土壤样本（1.06-26.93）中As的目标危害商（THQ > 1）。儿童的危害指数（HI）平均为4.00，大于成人（0.62），主要是由于砷和镉的贡献。它表明，通过典型的手到口的行为，儿童可能会发生非致癌风险。结肠生物可及性的调整将导致更准确的风险评估人类暴露于重金属污染土壤的口服摄入。
To identify the role of gut microbiota in human health risk assessment, the bioaccessibility of heavy metals in 14 soil samples were determined in simulated gastrointestinal fluids. Compared to the small intestinal phase, the bioaccessibility values of the colon phase varied, either increased by 3.5-fold for As, by 2.2-fold for Cr, and by 1.6-fold for Ni, or reduced by 4.4-fold for Cu, respectively. The colon incubation with adult gut microbiota yielded higher bioaccessibility value of As (1.3 times) and Fe (3.4 times) than that of the child in most soil samples. Colon bioaccessibility was about 60% greater of Cd for the adult and 30% higher of Cr for the child. Congruent data on the bioaccessibility of Cu and Ni was observed. In addition, correlation analysis indicated thatin vitrobioaccessibility was primarily related to total concentrations of heavy metals in soils, followed by soil pH and active Fe/Mn oxide. Significantly, risk assessment calculated based on colon bioaccessibility indicated that the target hazard quotient (THQ > 1) of As was presented in 3 soil samples for the adult (1.05–3.35) and in 9 soil samples for the child (1.06–26.93). The hazard index (HI) of the child was 4.00 on average, greater than that of the adult (0.62), primarily due to the contribution of As and Cd. It suggested non-carcinogenic risks are likely to occur in children through typical hand-to-mouth behavior. The adjustment of colon bioaccessibility will result in more accurate risk assessment of human exposure to heavy metals from oral ingestion of contaminated soils.