Enzymatic Glyco-Modification of Synthetic Membrane Systems.
Enzymatic Glyco-Modification of Synthetic Membrane Systems.
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DOI：
10.3390/biom13020335
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发表时间：
2023-02-09
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
本报告评估了可溶性糖基转移酶的能力，以修改糖脂组织在两个合成膜系统，有吸引力的模型，以模仿细胞膜：巨单层囊泡（GUV）和支持的脂质双层（SLB）。目的是在这些膜模型的表面合成Gb 3抗原（Galα1，4Galβ1，4Glcβ-Cer），一种癌症生物标志物。选择将来自UDP-Gal的半乳糖残基添加到含乳糖受体的可溶形式的LgtC。尽管比乳糖效率低，但在GUV和SLB上证明了LgtC利用乳糖基-神经酰胺作为受体的能力。使用滋贺毒素的B亚基作为Gb 3结合凝集素监测反应。石英晶体微天平与耗散分析表明，在膜表面的LgtC的瞬时结合是足够的生产性转化LacCer到Gb 3。分子动力学模拟提供了结构元素，以帮助合理化实验数据。
The present report assesses the capability of a soluble glycosyltransferase to modify glycolipids organized in two synthetic membrane systems that are attractive models to mimic cell membranes: giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs). The objective was to synthesize the Gb3 antigen (Galα1,4Galβ1,4Glcβ-Cer), a cancer biomarker, at the surface of these membrane models. A soluble form of LgtC that adds a galactose residue from UDP-Gal to lactose-containing acceptors was selected. Although less efficient than with lactose, the ability of LgtC to utilize lactosyl–ceramide as an acceptor was demonstrated on GUVs and SLBs. The reaction was monitored using the B-subunit of Shiga toxin as Gb3-binding lectin. Quartz crystal microbalance with dissipation analysis showed that transient binding of LgtC at the membrane surface was sufficient for a productive conversion of LacCer to Gb3. Molecular dynamics simulations provided structural elements to help rationalize experimental data.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
15
作者：
Chaubard, Jean-Luc;Krishnamurthy, Chithra;Yi, Wen;Smith, David F.;Hsieh-Wilson, Linda C.
通讯作者：
Hsieh-Wilson, Linda C.
相互作用熵：高效可靠计算蛋白质-配体结合自由能的新范式
影响因子：
15
作者：
Duan, Lili;Liu, Xiao;Zhang, John Z. H.
通讯作者：
Zhang, John Z. H.
影响因子：
5.5
作者：
Kociurzynski, Raisa;Makshakova, Olga N.;Roemer, Winfried
通讯作者：
Roemer, Winfried
DOI：
10.1016/j.bbrc.2011.04.062
发表时间：
2011-05-20
影响因子：
3.1
作者：
Alves, Claudia A.;Pedroso, Mariele M.;Faria, Ronaldo C.
通讯作者：
Faria, Ronaldo C.