Synchronous delivery systems composed of Au nanoparticles and stimuli-sensitive diblock terpolymer
Synchronous delivery systems composed of Au nanoparticles and stimuli-sensitive diblock terpolymer
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由金纳米颗粒和刺激敏感二嵌段三元共聚物组成的同步递送系统
DOI：
10.1007/s10856-004-5737-1
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Muhammed
中科院分区：
文献类型：
--
作者：
Y. Jo;D. K. Kim;M. Muhammed
本文提出了一种通过在聚[(N-异丙基丙烯酰胺-r-丙烯酰胺)-b-L-乳酸](PNAL)纳米球上直接自组装Au纳米粒子来构建同步递送系统的方法。为了获得两亲性二嵌段三元共聚物，通过米歇尔型加成反应将疏水性聚（L-乳酸）（PLLA）嵌段添加到聚（N-异丙基丙烯酰胺-r-丙烯酰胺）（PNA）嵌段中。通过控制异丙基丙烯酰胺（IPAAm）单体和丙烯酰胺（AAm）单体之间的比例，将下临界溶解温度（LCST）调节为35.6℃，接近体温，但高于聚（N-异丙基丙烯酰胺）（PNIPAAm）均聚物。使用这种两亲性二嵌段三元共聚物，通过乳液/蒸发技术制备了 PNAL 纳米球，然后由于 PNA 嵌段提供的氨基具有高亲和力，因此金纳米颗粒直接自组装在 PNAL 纳米球上。 Au@PNAL纳米球的“核心”位点可以负载各种亲脂性药物。此外，PNAL 纳米球“壳”域中的金纳米颗粒为缀合各种生物分子提供了最佳环境。因此，预计Au@PNAL杂化纳米球可用于“壳”域中的生物分子和“核”域中的各种治疗药物的同步递送。
A method to construct synchronous delivery systems via direct self-assembly of Au nanoparticles on the poly[(N-isopropylacrylamide-r-acrylamide)-b-L-lactic acid] (PNAL) nanospheres has been presented in this paper. To achieve amphiphilic diblock terpolymer, hydrophobic poly (L-lactic acid) (PLLA) block was added to poly(N-isopropylacrylamide- r-acrylamide) (PNA) block via Michel-type addition reaction. Lower critical solubility temperature (LCST) was modulated at 35.6 °C which is close to the body temperature, but higher than poly(N-isopropylacrylamide) (PNIPAAm) homopolymer by controlling the ratio between isopropylacrylamide (IPAAm) monomers and acrylamide (AAm) monomers. Using this amphiphilic diblock terpolymer, PNAL nanospheres were fabricated by emulsion/evaporation technique followed by direct self-assembly of Au nanoparticles on the PNAL nanospheres due to the high affinity of amino groups donated from PNA block. The ‘core’ site of Au@PNAL nanospheres can load various lyphophilic drugs. Moreover, Au nanoparticles in the ‘shell’ domain of PNAL nanospheres give optimal environment to conjugate various biomolecules. Therefore, it is expected that Au@PNAL hybrid nanospheres can be utilized in synchronous delivery of both biomolecules in the ‘shell’ domain and various therapeutic drugs in the ‘core’ domain.