In vivo evaluation of ocular inflammatory responses in experimental diabetes
In vivo evaluation of ocular inflammatory responses in experimental diabetes
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DOI：
10.1136/bjo.2004.061929
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发表时间：
2005-08-01
影响因子：
4.1
通讯作者：
Yoshimura, N
中科院分区：
文献类型：
--
作者：
Tamura, H;Kiryu, J;Yoshimura, N
目的：糖尿病患者可能存在对外源性或内源性刺激的异常炎症反应。本研究的目的是通过视网膜白细胞动力学在糖尿病大鼠炎症眼的糖尿病眼炎症反应进行评估。方法：糖尿病在Long-Evans大鼠诱导3周后，内毒素诱导的葡萄膜炎产生的足垫注射脂多糖（LPS）。结果：LPS致伤后12 h和48 h，白细胞滚动数呈双相性增加。白细胞在视网膜中的聚集数量在72小时达到高峰。与非糖尿病视网膜相比，糖尿病视网膜中滚动和聚集的最大白细胞数分别减少56.3%（P< 0.01）和46.7%（P< 0.0001）。在视网膜中的粘附分子，这是上调后LPS注射的mRNA表达水平，也低于糖尿病大鼠比在non-diabetic rats.Conclusion：这项研究是第一次显示内毒素诱导的炎症是在糖尿病眼的干扰，根据证据表明，白细胞-内皮细胞相互作用刺激的LPS在糖尿病视网膜被抑制。这些发现支持了糖尿病患者眼部炎症反应受损的理论。
Aims: Diabetic patients may have abnormal inflammatory reactions to foreign or endogenous stimuli. This study was designed to evaluate inflammatory reactions in the diabetic eye through retinal leucocyte dynamics in the inflamed eyes of diabetic rats.Methods: Three weeks after diabetes induction in Long-Evans rats, endotoxin induced uveitis was produced by footpad injection of lipopolysaccharide (LPS). After LPS injection, leucocyte behaviour was evaluated in vivo by acridine orange digital fluorography.Results: The number of rolling leucocytes increased in a biphasic manner at 12 hours and 48 hours. The number of leucocytes accumulating in the retina reached a peak at 72 hours. The maximal numbers of rolling and accumulating leucocytes in the diabetic retina decreased by 56.3% ( p< 0.01) and 46.7% ( p< 0.0001), respectively, compared with the non-diabetic retina. The levels of mRNA expression of adhesion molecules in the retina, which were upregulated after LPS injection, were also lower in diabetic rats than in non-diabetic rats.Conclusion: This study is the first to show that endotoxin induced inflammation is disturbed in the diabetic eye, based on evidence that the leucocyte-endothelial cell interactions stimulated by LPS were suppressed in the diabetic retina. These findings support the theory that ocular inflammatory reactions are impaired in diabetic patients.