Association of spin-labelled cardiolipin with dimyristoylphosphatidylcholine-substituted bovine heart cytochrome c oxidase. A generalized specificity increase rather than highly specific binding sites.
Association of spin-labelled cardiolipin with dimyristoylphosphatidylcholine-substituted bovine heart cytochrome c oxidase. A generalized specificity increase rather than highly specific binding sites.
复制标题
自旋标记心磷脂与二肉豆蔻酰磷脂酰胆碱取代的牛心细胞色素 c 氧化酶的关联。
DOI：
10.1016/0005-2736(85)90409-2
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Marsh
中科院分区：
文献类型：
--
作者：
G. Powell;P. Knowles;D. Marsh
牛心脏细胞色素氧化酶的内源性脂质已被二肉豆蔻酰磷脂酰胆碱使用胆酸盐介导的交换。脂质替代制剂含有少于1摩尔心磷脂每摩尔酶，并具有充分的氧化活性。自旋标记的心磷脂与这种脂质取代的细胞色素氧化酶制剂的协会已被测定使用ESR光谱。心磷脂的平均相对缔合常数是磷脂酰胆碱的5.4倍。在脂质交换过程中引入的未标记心磷脂含量增加的制剂上的测量表明，这种选择性对应于蛋白质上所有脂质缔合位点的特异性的普遍增加。
The endogeneous lipid of bovine heart cytochromecoxidase has been replaced by dimyristoylphosphatidylcholine using cholate-mediated exchange. The lipid-substituted preparation contained less than 1 mole cardiolipin per mole enzyme and possessed full oxidative activity. The association of spin-labelled cardiolipin with such lipid-substituted cytochrome oxidase preparations has been assayed using ESR spectroscopy. An average relative association constant 5.4-times that for phosphatidylcholine is obtained for cardiolipin. Measurements on preparations with increasing contents of unlabelled cardiolipin, introduced during lipid exchange, reveal that this selectivity corresponds to a generalized increase in specificity for all lipid association sites on the protein.