Isolation of lead-resistant Arthrobactor strain GQ-9 and its biosorption mechanism
Isolation of lead-resistant Arthrobactor strain GQ-9 and its biosorption mechanism
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DOI：
10.1007/s11356-017-0694-7
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发表时间：
2017-11
影响因子：
5.8
通讯作者：
Tianqi Wang;Jun Yao;Zhi-min Yuan;Yue Zhao;Fei Wang;Huilun Chen
中科院分区：
文献类型：
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作者：
Tianqi Wang;Jun Yao;Zhi-min Yuan;Yue Zhao;Fei Wang;Huilun Chen
本研究中，抗铅细菌Arthroplasticsp.从重金属污染土壤中筛选出对镉、锌、铜具有抗性的菌株GQ-9。微量热分析应用于铅（II）胁迫下的菌株的微生物活性进行评估，并表明GQ-9的铅（II）胁迫下的微生物活性比非抗性菌株强。吸附实验结果表明，GQ-9吸附Pb（II）的最佳条件为pH 5.5、投加量1.2 g L−1、初始Pb（II）浓度100 mg L− 1，最大吸附量为17.56 mg g−1。吸附-脱附分析表明，GQ-9细胞可以将56.60%的吸附Pb（II）离子固定在细胞壁上。FTIR分析表明，GQ-9细胞壁上的羟基、羧基、氨基、腈基、巯基以及酰胺I、酰胺II带参与了Pb（II）离子的络合。本研究表明，耐铅细菌GQ-9有潜力进一步开发一种有效的和生态友好的重金属生物修复吸附剂。
In this study, lead-resistant bacteriumArthrobactersp. GQ-9 with a resistant capability to cadmium, zinc, and copper was isolated from a heavy metal polluted soil. Microcalorimetry analysis was applied to assess the strain’s microbial activity under Pb(II) stress and suggested that GQ-9’s microbial activities under Pb(II) stress were stronger than a non-resistant strain. Biosorption batch experiments revealed that the optimal condition for adsorption of Pb(II) by GQ-9 was pH 5.5, a biomass dosage of 1.2 g L−1, and an initial Pb(II) concentration of 100 mg L−1with a maximum biosorption capacity of 17.56 mg g−1.Adsorption-desorption experiments and Fourier transform infrared spectroscopy (FTIR) analysis were applied to elucidate the biosorption mechanisms. Adsorption-desorption analysis showed that GQ-9 cells could sequester 56.60% of the adsorbed Pb(II) ions on the cell wall. FTIR analysis suggested that hydroxyl, carboxyl, amino, nitrile, and sulfhydryl groups and amide I, amide II bands on the GQ-9 cell wall participated in the complexation of Pb(II) ions. The present study illustrates that the lead-resistant bacteria GQ-9 has the potential for further development of an effective and ecofriendly adsorbent for heavy metal bioremediation.