Mechanisms of cisplatin resistance in clear cell carcinoma of the ovary
Mechanisms of cisplatin resistance in clear cell carcinoma of the ovary
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DOI：
10.1159/000065067
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Terakawa, N
中科院分区：
文献类型：
--
作者：
Itamochi, H;Kigawa, J;Terakawa, N
卵巢透明细胞癌（CCC）对铂类化疗的耐药与预后不良有关。然而，CCC对铂的耐药性的机制尚未被理解。本研究旨在阐明CCC细胞对顺铂耐药的机制。本研究使用了11个CCC和5个浆液性腺癌（SAC）细胞系。CCC细胞对CDDP的IC 50为1.3 - 18.0 μ M，SAC细胞为2.2 - 13.0 μ M。CCC细胞多药耐药相关蛋白表达与顺铂敏感性无相关性。相比之下，CCC细胞的倍增时间显著长于SAC细胞（61.4 vs. 29.8 h）。观察到S期分数与对CDDP的反应之间的显著负相关（r = 0.647，p < 0.05）。本研究提示CCC对顺铂的耐药性可能与细胞增殖能力低下有关。版权所有（C）2002 S. Karger AG，巴塞尔。
Resistance of clear cell carcinoma (CCC) of the ovary to platinum-based chemotherapy is associated with a poor prognosis. However, the mechanism underlying the resistance of CCC to platinum has not yet been understood. We conducted the present study to clarify the mechanism of cisplatin (CDDP) resistance in CCC cells. Eleven CCC and 5 serous adenocarcinoma (SAC) cell lines were used in this study. The IC50 to CDDP ranged from 1.3 to 18.0 muM for CCC cells and from 2.2 to 13.0 muM for SAC cells. There was no correlation between multidrug resistance-associated protein expression and the sensitivity to CDDP in CCC cells. In contrast, the doubling time for CCC cells was significantly longer than that for SAC cells (61.4 vs. 29.8 h). A significant reverse correlation between the S-phase fraction and the response to CDDP was observed (r = 0.647, p < 0.05). The present study suggests that the resistance of CCC to CDDP may be caused by low cell proliferation. Copyright (C) 2002 S. Karger AG, Basel.