A comparison of balloon injury models of endovascular lesions in rat arteries.
A comparison of balloon injury models of endovascular lesions in rat arteries.
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DOI：
10.1186/1471-2261-2-16
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发表时间：
2002-09-27
影响因子：
2.1
通讯作者：
van Urk, Hero
中科院分区：
文献类型：
--
作者：
Gabeler, Edward E E;van Hillegersberg, Richard;van Urk, Hero
背景：大鼠颈动脉（CCA）球囊损伤（BI）被广泛用于研究内膜增生（IH）和管腔直径减小（LD），但CCA的小直径阻碍了血管内治疗的评估。因此，我们在主动脉（AA）和髂动脉（CIA）验证BI，并将其与CCA进行比较。方法：大鼠接受BI或假手术（对照组）。直接或在随访1、2、3、4和16周时进行光镜评价。比较IH面积和LD的变化（16周时的LD减去BI后的LD）。结果：在16周（NS）时，bi组IH面积分别增加到0.14 +/- 0.08 mm2 （CCA）、0.14 +/- 0.03 mm2 （CIA）和0.12 +/- 0.04 mm2 （AA）。16周时，LD为0.49 +/- 0.07 mm (CCA)，而CIA和AA分别为0.22 +/- 0.07 mm和0.07 +/- 0.10 mm （p < 0.05）。CIA组血管收缩性重构（CVR =壁周损失合并LD降低）为-0.17 +/- 0.05 mm，而CCA和AA组没有。对照组未见IH、LD和CVR下降。结论：BI导致：(1)IH导致CCA LD降低，(2)IH和CVR导致CIA LD降低，(3)AA LD无变化，(4)AA LD无变化。所有动脉的IH发展情况比较，(5)。与CIA和AA相比，CCA没有血管，(6)。CIA模型结合了2f血管内导管的良好通路和BI后IH和CVR导致的LD降低。
BACKGROUND: Balloon injury (BI) of the rat carotid artery (CCA) is widely used to study intimal hyperplasia (IH) and decrease in lumen diameter (LD), but CCA's small diameter impedes the evaluation of endovascular therapies. Therefore, we validated BI in the aorta (AA) and iliac artery (CIA) to compare it with CCA.METHODS: Rats underwent BI or a sham procedure (control). Light microscopic evaluation was performed either directly or at 1, 2, 3, 4 and 16 weeks follow-up. The area of IH and the change in LD (LD at 16 weeks minus LD post BI) were compared.RESULTS: In the BI-groups the area of IH increased to 0.14 +/- 0.08 mm2 (CCA), 0.14 +/- 0.03 mm2 (CIA) and 0.12 +/- 0.04 mm2 (AA) at 16 weeks (NS). The LD decreased with 0.49 +/- 0.07 mm (CCA), compared to 0.22 +/- 0.07 mm (CIA) and 0.07 +/- 0.10 mm (AA) at 16 weeks (p < 0.05). The constrictive vascular remodelling (CVR = wall circumference loss combined with a decrease in LD) was -0.17 +/- 0.05 mm in CIA but absent in CCA and AA. No IH, no decrease in LD and no CVR was seen in the control groups.CONCLUSIONS: BI resulted in: (1.) a decrease in LD in CCA due to IH, (2.) a decrease in LD in CIA due to IH and CVR, (3.) no change in LD in AA, (4.) Comparable IH development in all arteries, (5.) CCA has no vasa vasorum compared to CIA and AA, (6.) The CIA model combines good access for 2 F endovascular catheters with a decrease in LD due to IH and CVR after BI.