Inverted papillomas of the paranasal sinuses and the nasal cavity: DNA indices and HPV infection
Inverted papillomas of the paranasal sinuses and the nasal cavity: DNA indices and HPV infection
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DOI：
10.2500/105065897782537160
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发表时间：
1997-03-01
期刊：
影响因子：
--
通讯作者：
Mann, WJ
中科院分区：
文献类型：
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作者：
Bernauer, S;Welkoborsky, HJ;Mann, WJ
内翻性乳头状瘤是一种罕见的鼻腔及鼻窦肿瘤。这些肿瘤通常被认为是良性病变;然而，恶性转化发生在高达13%的病例中。在本回顾性研究中，标本从21例鼻窦和鼻腔内翻性乳头状瘤进行了调查，以分析遗传不平衡的分布和分类染色体非整倍体在这些肿瘤作为其生物学行为的预测。目前，人乳头状瘤病毒（HPV）被认为是上呼吸消化道粘膜组织良性和恶性肿瘤的诱导和促进因子。迄今为止，已经通过核苷酸序列的差异鉴定了80多种HPV类型。几种类型（例如，HPV 16、HPV 18）与癌症发生风险增加有关。其他类型（例如，HPV 6/11）在分层粘膜的良性病变中更常见。我们用通用和特异性引物聚合酶链反应检测HPV基因组。使用来自L1主包膜基因的简并引物，在21例中的7例中观察到正确大小的HPV DNA片段的扩增。在一个标本中，其中一个与鳞状细胞癌相关的内翻性乳头状瘤被组织学证实，HPV 18特异性引物检测到HPV 18基因组。其中观察到HPV DNA片段扩增的肿瘤显示出最高的增殖评分沿着最高的非整倍体细胞率，其通过定量DNA分析检测到。
Inverted papillomas are rare tumors of the nasal cavity and paranasal sinuses. These tumors are commonly regarded as benign lesions; nevertheless, malignant transformation occurs in up to 13% of cases. In the present retrospective study, specimens from 21 patients with inverted papillomas of the paranasal sinuses and the nasal cavity were investigated in order to analyze the distribution of genetic imbalance and to classify chromosomal aneuploidy in these tumors as a predictor for their biological behavior. Currently, human papilloma viruses (HPVs) are accepted as tumor-inducing and -promoting agents involved in benign and malignant tumors of mucosal tissues in the upper aerodigestive tract. To date more than 80 HPV types have been identified by differences in the nucleotide sequences. Several types (e.g., HPV16, HPV18) are associated with an increased risk for developing cancer. Other types (e.g., HPV6/11) are identified more frequently in benign lesions of stratified mucous membranes. We detected HPV genomes by universal and specific primer polymerase chain reaction. Using degenerated primers derived from the L1 major envelope gene, an amplification of HPV DNA fragments of the correct size was observed in 7 of 21 cases. In one specimen, where an inverted papilloma associated with a squamous cell carcinoma was histologically confirmed, HPV18-specific primers detected the HPV18 genome. The tumors in which an amplification of HPV DNA fragments were observed showed the highest proliferation scores along with the highest rate of aneuploid cells detected by quantitative DNA analysis.