Development of an AND Logic-Gate-Type Fluorescent Probe for Ratiometric Imaging of Autolysosome in Cell Autophagy
Development of an AND Logic-Gate-Type Fluorescent Probe for Ratiometric Imaging of Autolysosome in Cell Autophagy
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DOI：
10.1002/chem.201405686
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发表时间：
2015-01-26
影响因子：
4.3
通讯作者：
Ojida, Akio
中科院分区：
文献类型：
--
作者：
Takashima, Ippei;Kawagoe, Ryosuke;Ojida, Akio
两种生物事件的同时检测有助于对特定生物功能进行高度选择性和灵敏的分析。在本文中，我们报道了一种 AND 逻辑门型荧光探针，它可以同时感知活细胞中的两个生物事件：H2O2 积累和酸化。该探针表现出独特的荧光传感机制，其中呫吨荧光团被H2O2氧化转化为呫吨酮衍生物，从而产生明显的双发射变化。这种转化在弱酸性条件下显着加速，从而能够选择性且灵敏地检测酸性细胞室中 H2O2 的产生。这种独特的传感特性已成功应用于选择性线粒体自噬（mitophagy）中自溶酶体形成的比例荧光成像，这凸显了这种新型探针在自噬研究中的实用性。
The concomitant detection of two biological events facilitates the highly selective and sensitive analysis of specific biological functions. In this article, we report an AND logic-gate-type fluorescent probe that can concurrently sense two biological events in living cells: H2O2 accumulation and acidification. The probe exhibits a unique fluorescence sensing mechanism, in which a xanthene fluorophore is oxidatively transformed to a xanthone derivative by H2O2, thereby resulting in a clear dual-emission change. This trans-formation is significantly accelerated under weak acidic conditions, which enables the selective and sensitive detection of H2O2 production in an acidic cellular compartment. This unique sensing property was successfully applied to the ratiometric fluorescence imaging of autolysosome formation in selective mitochondrial autophagy (mitophagy), which highlights the utility of this novel probe in autophagy research.