Phylogeny of the bee genus Halictus (Hymenoptera: Halictidae) based on parsimony and likelihood analyses of nuclear EF-1α sequence data
Phylogeny of the bee genus Halictus (Hymenoptera: Halictidae) based on parsimony and likelihood analyses of nuclear EF-1α sequence data
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DOI：
10.1006/mpev.1999.0670
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发表时间：
1999-12-01
影响因子：
4.1
通讯作者：
Packer, L
中科院分区：
文献类型：
--
作者：
Danforth, BN;Sauquet, H;Packer, L
我们基于延伸因子-批次DNA序列数据的简约和最大似然(ML)分析，研究了Halictus属内更高级别的系统发育关系。我们的数据集包括41个OTUS，代表35种盐蜂属，它们来自不同的外群属和广为人知的三个盐蜂亚属(Halictus S.S.，Seladonia和Vestitohalictus)。我们分析了跨越三个外显子和两个内含子的1513个总比对的核苷酸位点。对整个数据集进行等权简约分析，得到144棵同样简约的树。本分析所支持的主要结论(在随后的所有分析中都得到了支持)包括：(1)海燕是Halictus s.l.的姊妹群，(2)Halictus s.l。(4)Michener群1和3是单系群，(5)Michener群1使群2是单系群。为了解决Halictus内部的基本关系，我们应用了简约下的各种加权方案(逐次逼近、字符加权和隐含加权)，并在17种序列进化模型下使用了ML。加权节俭产生了相互矛盾的结果，但总体上支持这样的假设，即Seladonia+Vestitohalictus是Michener的第三组的姐妹，并使Halictus S.S.有过敏症。使用具有特定部位比率的GTR模型进行的ML分析支持另一种假设：Seladonia+Vestitohalictus是Halictus S.S.的姊妹种。我们将社会行为映射到ML和简约下得到的树上，以重建可能的社会进化的历史模式。我们的结果是明确的：Halictus属的祖先状态是幸福的。在我们分析的物种中，向独居行为的逆转至少发生了四次。(C)1999年学术出版社
We investigated higher-level phylogenetic relationships within the genus Halictus based on parsimony and maximum likelihood (ML) analysis of elongation factor-lot DNA sequence data. Our data set includes 41 OTUs representing 35 species of halictine bees from a diverse sample of outgroup genera and from the three widely recognized subgenera of Halictus (Halictus s.s., Seladonia, and Vestitohalictus). We analyzed 1513 total aligned nucleotide sites spanning three exons and two introns. Equal-weights parsimony analysis of the overall data set yielded 144 equally parsimonious trees. Major conclusions supported in this analysis land in all subsequent analyses) included the following: (1) Thrincohalictus is the sister group to Halictus s.l., (2) Halictus s.l. is monophyletic, (3) Vestitohalictus renders Seladonia paraphyletic but together Seladonia + Vestitohalictus is monophyletic, (4) Michener's Groups 1 and 3 are monophyletic, and (5) Michener's Group 1 renders Group 2 paraphyletic. In order to resolve basal relationships within Halictus we applied various weighting schemes under parsimony (successive approximations character weighting and implied weights) and employed ML under 17 models of sequence evolution. Weighted parsimony yielded conflicting results but, in general, supported the hypothesis that Seladonia + Vestitohalictus is sister to Michener's Group 3 and renders Halictus s.s. paraphyletic. ML analyses using the GTR model with site-specific rates supported an alternative hypothesis: Seladonia + Vestitohalictus is sister to Halictus s.s. We mapped social behavior onto trees obtained under ML and parsimony in order to reconstruct the likely historical pattern of social evolution. Our results are unambiguous: the ancestral state for the genus Halictus is eusociality. Reversal to solitary behavior has occurred at least four times among the species included in our analysis. (C) 1999 Academic Press