Perception of Agrobacterium tumefaciens flagellin by FLS2XL confers resistance to crown gall disease
Perception of Agrobacterium tumefaciens flagellin by FLS2XL confers resistance to crown gall disease
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DOI：
10.1038/s41477-019-0578-6
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发表时间：
2020-01-01
期刊：
影响因子：
18
通讯作者：
Felix, Georg
中科院分区：
文献类型：
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作者：
Fuerst, Ursula;Zeng, Yi;Felix, Georg
植物 (1) 和动物 (2) 的先天免疫系统都会感知细菌鞭毛，从而引发抵抗力。高等植物共有免疫受体 FLAGELLIN-SENSING 2 (FLS2)(3)，它通过其最保守的表位 flg22 检测鞭毛蛋白。根癌农杆菌在许多作物中引起冠瘿病，其 flg22 表位高度分化，并且可以逃避迄今为止研究的植物的免疫检测。我们询问，作为“捉迷藏”游戏的下一步，是否有植物物种已经进化出对农杆菌的伪装 flg22(Atum) 具有特异性的免疫受体。在野生葡萄品种 Vitis riparia 中，我们发现了 FLS2(XL)，这是一种以前未知的 FLS2 形式，它对典型 flg22 和 flg22(Atum) 具有极高的敏感性。以烟草中的异位表达为例，FLS2(XL) 可以限制由根癌农杆菌引起的冠瘿病。免疫受体 FLS2 感知来自许多细菌病原体的鞭毛蛋白表位，但不能感知来自众所周知的根癌农杆菌的鞭毛蛋白表位。作者在野生葡萄品种中发现了一种天然的 FLS2，它可以感知这种修饰的鞭毛蛋白。
Bacterial flagella are perceived by the innate immune systems of plants(1) and animals(2) alike, triggering resistance. Common to higher plants is the immunoreceptor FLAGELLIN-SENSING 2 (FLS2)(3), which detects flagellin via its most conserved epitope, flg22. Agrobacterium tumefaciens, which causes crown gall disease in many crop plants, has a highly diverged flg22 epitope and evades immunodetection by plants so far studied. We asked whether, as a next step in this game of 'hide and seek', there are plant species that have evolved immunoreceptors with specificity for the camouflaged flg22(Atum) of A. tumefaciens. In the wild grape species Vitis riparia, we discovered FLS2(XL), a previously unknown form of FLS2, that provides exquisite sensitivity to typical flg22 and to flg22(Atum). As exemplified by ectopic expression in tobacco, FLS2(XL) can limit crown gall disease caused by A. tumefaciens.Immune receptor FLS2 perceives flagellin epitopes from many bacterial pathogens, but not from well-known Agrobacterium tumefaciens. The authors find a natural FLS2 in a wild grape species that can perceive this modified flagellin.