Salivary microflora and mode of delivery: a prospective case control study.
Salivary microflora and mode of delivery: a prospective case control study.
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DOI：
10.1186/s12903-015-0142-3
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发表时间：
2015-12-03
期刊：
影响因子：
2.9
通讯作者：
Twetman S
中科院分区：
文献类型：
--
作者：
Boustedt K;Roswall J;Dahlén G;Dahlgren J;Twetman S
以前的横断面研究表明，交付方式可以影响口腔微生物群落的组成。这项前瞻性研究的目的是比较唾液定植在阴道分娩的儿童和剖腹产分娩的儿童（剖腹产）在他们的生命的第一个6个月。研究组包括149名连续入选的婴儿，经阴道分娩（n = 96）或剖腹产（n = 53），这些婴儿在父母同意后自愿参加。唾液样本在出生后2天内收集，然后在1，3和6个月后收集。在分娩后6个月获得母亲的唾液样本。父母被要求在基线和整个研究期间完成一份关于社会经济因素、生活方式和卫生的问卷。所有样品用13种预先确定的细菌探针进行分析，使用棋盘DNA-DNA杂交。各组在基线时所有相关背景因素均保持平衡。革兰氏阳性链球菌（S. mitis、细叶山蚂蝗S.唾液腺）在两组中均显示出最高的计数，但在阴道分娩组中观察到更大的多样性。A. naeslundi、黑腹拟步行虫A. dontolytics、F. nucleatum和L.仅在阴道分娩的婴儿中检测到流涎。S. sanguinis，S.戈尔多尼河denticariosa和B.两组牙本质均随年龄增长而增加，但剖宫产组牙本质患病率明显低于剖宫产组（p < 0.05）。母亲和她们的后代在唾液微生物谱方面存在联系。唾液中的微生物组成在生命的前六个月内因分娩方式而异。
Previous cross-sectional studies have suggested that the mode of delivery can influence the composition of oral microflora. The aim of this prospective study was to compare the salivary colonization in vaginally delivered children with children delivered by Caesarian section (C-section) during their first 6 months of life. The study group consisted of 149 consecutively enrolled infants, delivered either vaginally (n = 96) or by C-section (n = 53) that volunteered after consent of their parents. Saliva samples were collected within 2 days after birth and then after 1, 3, and 6 months. A saliva sample from the mothers was obtained 6 months after delivery. The parents were asked to complete a questionnaire on socioeconomic factors, lifestyle, and hygiene at baseline and throughout the study period. All samples were analyzed with 13 pre-determined bacterial probes using checkerboard DNA-DNA hybridization. The groups were balanced at baseline concerning all relevant background factors. Gram-positive streptococci (S. mitis, S. salivarius) displayed the highest counts in both groups but a greater diversity was observed in the vaginally delivered group. A. naeslundi, A. odontolytics, F. nucleatum and L. salivarius were only detected among the vaginally delivered infants. The prevalence of S. sanguinis, S. gordoni, R. denticariosa, and B. dentinum increased by age in both groups but the prevalence was significantly lower in the C-section group (p < 0.05). There was a link between the mothers and their offspring’s concerning the salivary microbial profile. The microbial composition in saliva differs by the mode of delivery during the first six months of life.