Serotonin 2A receptor mRNA levels in the neonatal dopamine-depleted rat striatum remain upregulated following suppression of serotonin hyperinnervation
Serotonin 2A receptor mRNA levels in the neonatal dopamine-depleted rat striatum remain upregulated following suppression of serotonin hyperinnervation
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DOI：
10.1016/s0165-3806(99)00066-8
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发表时间：
1999-08-05
期刊：
影响因子：
--
通讯作者：
Walker, PD
中科院分区：
文献类型：
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作者：
Basura, GJ;Walker, PD
用6-羟基多巴胺(6-OHDA)耗竭新生大鼠双侧多巴胺(>；98%)60天后，5-MT含量增加一倍，5-HT2A受体mRNA表达增加54%。为了确定多巴胺耗竭后纹状体5-HT2A受体基因表达上调是否依赖于5-羟色胺水平的升高，新生大鼠接受了6-OHDA和5，7-二羟色胺(5，7-DHT)的双重注射，其抑制5-羟色胺的含量约为90%。在这些6-OHDA/5，7-DHT处理的大鼠中，纹状体5-HT2A受体mRNA的表达仍然升高(比赋形剂对照组高87%)。5-HT2a受体mRNA表达的比较分析显示，各实验组均未见明显变化。这些结果表明，DA耗竭大鼠上调的5-HT2A受体的生物合成不依赖于随后的5-羟色胺的过度神经支配。(C)1999 Elsevier Science B.V.保留所有权利。
Sixty days after bilateral dopamine (DA) depletion (> 98%) with 6-hydroxydopamine (6-OHDA) in neonatal rats, serotonin (5-MT) content doubled and 5-HT2A receptor mRNA expression rose 54% within the rostral striatum. To determine if striatal 5-HT2A receptor mRNA upregulation is dependent on increased 5-HT levels following DA depletion, neonatal rats received dual injections of 6-OHDA and 5,7-dihydroxytryptamine (5,7-DHT) which suppressed 5-HT content by approximately 90%. In these 6-OHDA/5,7-DHT-treated rats, striatal 5-HT2A receptor mRNA expression was still elevated (87% above vehicle controls). Comparative analysis of 5-HT2A receptor mRNA expression yielded no significant changes in any experimental group. These results demonstrate that upregulated 5-HT2A receptor biosynthesis in the DA-depleted rat is not dependent on subsequent 5-HT hyperinnervation. (C) 1999 Elsevier Science B.V. All rights reserved.