Nicking-Assisted Reactant Recycle To Implement Entropy-Driven DNA Circuit
Nicking-Assisted Reactant Recycle To Implement Entropy-Driven DNA Circuit
复制标题
切口辅助反应物回收以实现熵驱动 DNA 电路
DOI：
10.1021/jacs.9b07521
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发表时间：
2019
影响因子：
15
通讯作者：
Yan Hao
中科院分区：
文献类型：
--
作者：
Zhang Cheng;Wang Zhiyu;Liu Yan;Yang Jing;Zhang Xinxin;Li Yifan;Pan Linqiang;Ke Yonggang;Yan Hao
合成催化 DNA 电路因其强大的模块化特性而成为分子编程的重要信号放大工具。在催化回路中，反应物回收操作对于促进连续过程至关重要。因此，需要开发新的反应物回收方法并提高熵驱动DNA电路反应的可回收性。在这里，我们描述了熵驱动 DNA 电路中反应物的切口辅助回收策略的实施，其中双链 DNA 废物能够恢复为可以参与下一个反应循环的活性成分。构建并分析了不同设计的单层电路和多个双层电路。在反应过程中，单层催化回路可以消耗多余的燃料 DNA 链，而不会耗尽门组件。两层电路的回收发生在燃料 DNA 消化期间，但不在下游触发器的释放期间。该策略提供了一种简单而通用的方法，用于为分子编程和合成生物学创建更有效的熵驱动 DNA 电路。
Synthetic catalytic DNA circuits are important signal amplification tools for molecular programming due to their robust and modular properties. In catalytic circuits, the reactant recycling operation is essential to facilitate continuous processes. Therefore, it is desirable to develop new methods for the recycling of reactants and to improve the recyclability in entropy-driven DNA circuit reactions. Here, we describe the implementation of a nicking-assisted recycling strategy for reactants in entropy-driven DNA circuits, in which duplex DNA waste products are able to revert into active components that could participate in the next reaction cycle. Both a single-layered circuit and multiple two-layered circuits of different designs were constructed and analyzed. During the reaction, the single-layered catalytic circuit can consume excess fuel DNA strands without depleting the gate components. The recycling of the two-layered circuits occurs during the fuel DNA digestion but not during the release of the downstream trigger. This strategy provides a simple yet versatile method for creating more efficient entropy-driven DNA circuits for molecular programming and synthetic biology.