Cytogenetics of the chronic myeloid leukemia-derived cell line K562: Karyotype clarification by multicolor fluorescence in situ hybridization, comparative genomic hybridization, and locus-specific fluorescence in situ hybridization
Cytogenetics of the chronic myeloid leukemia-derived cell line K562: Karyotype clarification by multicolor fluorescence in situ hybridization, comparative genomic hybridization, and locus-specific fluorescence in situ hybridization
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DOI：
10.1016/s0165-4608(99)00169-7
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发表时间：
2000-04-01
影响因子：
--
通讯作者：
Nacheva, EP
中科院分区：
文献类型：
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作者：
Gribble, SM;Roberts, I;Nacheva, EP
慢性粒细胞白血病（CML）从慢性期向急性期的转化经常伴随着额外的染色体改变。广泛的染色体G显带研究表明，继发性变化是非随机的，通常包括三体8，等染色体17 q，三体19，或费城染色体的额外拷贝。除了这些次级染色体的变化，复杂的结构重排经常发生，以形成标记结构，仍然是传统的G-显带和sis的身份。CML衍生的细胞系K562自1975年首次建立以来一直是研究中使用的模型。然而，K562和K562 pe仍然不完整，标记结构从未被完全描述过。荧光原位杂交（FISH）技术的最新进展为基于24色染色体涂染（M-FISH）的染色体分类提供了可能。在这项研究中，我们报告了一个澄清的K562的染色体组型获得了以下分子细胞遗传学技术的组合：比较基因组杂交（CGH），FISH定位使用位点特异性探针，和FISH。多色FISH已经确定了该细胞系中的标记结构。本研究证实K562的特征标记染色体为der（18）t（1;18）。多色FISH证实了C-分带部分鉴定的标记结构为der（6）t（6;6）、der（17）t（9;17）、der（21）t（1;21）、der（5）t（5;6）。此外，M-FISH还揭示了一个缺失的20 q和一个复杂的小中部着丝粒标记，该标记来自染色体1、6和20。一个隐藏的重排之间的染色体12和21，产生了一个结构，看起来像一个正常的染色体12同源的G-显带分析。最后，M-FISH检测到13号染色体插入两个近端着丝粒标记的区域。(C)Elsevier Science Inc.，2000. All rights reserved.
The transformation of chronic myeloid leukemia (CML) from a chronic phase to on acute phase is frequently accompanied by additional chromosome changes. Extensive chromosome G-banded studies have revealed the secondary changes are nonrandom and frequently include trisomy 8, isochromosome 17q, trisomy 19, or an extra copy of the Philadelphia chromosome. In addition to these secondary chromosome changes, complex structural rearrangements often occur to form marker structures that remain unidentified by conventional G-banded and sis. The CML-derived cell line, K562, has been model used in research since it was originally established in 1975. The K562 K562 pe however, has remained incomplete, and marker structures have never been fully described. Recent advances in fluorescence in situ hybridization (FISH) technology have introduced the possibility of chromosome classification based on 24-color chromosome painting (M-FISH). In this study, we report a clarified karotype for K562 obtained by a combination of the following molecular cytogenetic techniques: comparative genomic hybridization (CGH), FISH mapping using locus-specific probes, and FISH. Multi-color FISH has identified the marker structures in this cell line. The characteristic marker chromosome in K562 has been confirmed bq this study to be a der(18)t(1;18). Multicolor FISH confirmed the identity of marker structures partially identified by C-banding as der(6)t(6;6), der(17)t(9;17), der(21)t(1;21), der(5) t(5;6). In addition M-FISH has revealed a deleted 20q and a complex small metacentric marker corn prised of material from chromosomes 1, 6, and 20. A cryptic rearrangement was revealed between chromosomes 12 and 21 that produced a structure that looks like a normal chromosome 12 homologue by G-banding analysis. Finally, M-FISH detected regions from chromosome 13 intercalated into two acrocentric markers. (C) Elsevier Science Inc., 2000. All rights reserved.