ROLE OF THE NA+,K+ PUMP IN HERPES-SIMPLEX TYPE-1-INDUCED CELL-FUSION - MELITTIN CAUSES SPECIFIC REVERSION OF SYNCYTIAL MUTANTS WITH THE SYN1 MUTATION TO SYN+ (WILD-TYPE) PHENOTYPE
ROLE OF THE NA+,K+ PUMP IN HERPES-SIMPLEX TYPE-1-INDUCED CELL-FUSION - MELITTIN CAUSES SPECIFIC REVERSION OF SYNCYTIAL MUTANTS WITH THE SYN1 MUTATION TO SYN+ (WILD-TYPE) PHENOTYPE
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DOI：
10.1006/viro.1993.1510
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发表时间：
1993-10-01
期刊：
影响因子：
3.7
通讯作者：
KOUSOULAS, KG
中科院分区：
文献类型：
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作者：
BAGHIAN, A;KOUSOULAS, KG
为了评价Na+、K+泵和离子梯度在病毒诱导的细胞融合中的重要性，我们研究了蜂毒肽（melittin）对HSV-1合胞体突变体引起的细胞融合的影响。蜂毒肽是蜜蜂毒液中发现的26个氨基酸的生物活性肽。蜂毒肽抑制HSV-1突变病毒mP（MP）、科斯（syn 20）和科斯（FFV 3）引起的Vero细胞融合。然而，它不影响由突变体HFEM（tsB 5）或具有糖蛋白B突变的KOSamb 1511 -7引起的细胞融合。蜂毒肽可使syn 1突变病毒空斑特异性逆转为synn+（野生型）空斑形态，并抑制病毒的吸附和穿透。抑制Na ~+、K ~+泵活性，抑制~ 3 H-哇巴因与Na ~+、K ~+泵的结合。感染的Vero细胞的Na+，K+泵活性相比，模拟感染的细胞显着降低。哇巴因是Na+、K+泵的特异性抑制剂，可抑制所有合胞病毒株引起的Vero细胞融合。
To evaluate the importance of the Na+,K+pump and ionic gradients in virus-induced cell fusion, we investigated the effects of melittin, a 26 amino acid bioactive peptide found in honey bee venom, on cell fusion caused by HSV-1 syncytial mutants. Melittin inhibited fusion of Vero cells caused by HSV-1 mutant viruses mP(MP), KOS (syn20) and KOS (FFV3) containing the syncytial mutationsyn1 in glycoprotein K. However, it did not affect cell fusion caused by mutants HFEM(tsB5) or KOSamb1511-7 with mutations in glycoprotein B. Melittin caused specific reversion ofsyn1 mutant virus plaques tosyn+(wild-type) plaque morphology, and inhibited virus adsorption and penetration. It also inhibited the Na+,K+pump activity, and the binding of3H-ouabain to the Na+,K+pump of infected Vero cells. The Na+,K+pump activity of infected Vero cells in comparison to mock-infected cells was significantly decreased. Ouabain, a specific inhibitor of the Na+,K+pump, inhibited fusion of Vero cells caused by all syncytial virus strains.