Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors
Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors
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DOI：
10.1007/s00253-001-0871-x
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发表时间：
2002-03
影响因子：
5
通讯作者：
A. Ganguli;A. Tripathi
中科院分区：
文献类型：
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作者：
A. Ganguli;A. Tripathi
比较了铜绿假单胞菌A2 Chr在分批培养中的铬还原能力，其中细胞包埋在透析囊中，并与细胞固定在琼脂糖-海藻酸盐膜结合旋转生物接触器中。在所有三个系统中，最大Cr（VI）减少发生在10 mg Cr（VI）/l。而在50 mg Cr（VI）/l浓度下，仅减少了16%的总Cr（VI），以2小时间隔用10 mg铬酸盐/l进行5次加标，导致50 mg Cr（VI）/l总输入量减少96%。因此，通过以较低Cr（VI）浓度呼吸避免Cr（VI）对细胞的毒性来实现最大Cr（VI）减少。在10毫克铬（VI）/升，在透析截留细胞中的铬酸盐还原的模式几乎是类似的分批培养和86%的细菌还原铬被保留在透析囊内。然而，在电镀废水中含有100毫克铬（VI）/升，铬（VI）的量减少的细胞固定在琼脂糖-藻酸盐生物膜的两倍和三倍的量减少分批培养和细胞截留在透析囊，分别。
The chromate-reducing ability ofPseudomonas aeruginosaA2Chr was compared in batch culture, with cells entrapped in a dialysis sac, and with cells immobilized in an agarose-alginate film in conjunction with a rotating biological contactor. In all three systems, the maximum Cr(VI) reduction occurred at 10 mg Cr(VI)/l. Whereas at 50 mg Cr(VI)/l concentration, only 16% of the total Cr(VI) was reduced, five spikings with 10 mg chromate/l at 2-h intervals led to 96% reduction of the total input of 50 mg Cr(VI) /l. Thus maximum Cr(VI) reduction was achieved by avoiding Cr(VI) toxicity to the cells by respiking with lower Cr(VI) concentrations. At 10 mg Cr(VI)/l, the pattern of chromate reduction in dialysis-entrapped cells was almost similar to that of batch culture and 86% of the bacterially reduced chromium was retained inside the dialysis sac. In electroplating effluent containing 100 mg Cr(VI)/l, however, the amount of Cr(VI) reduced by the cells immobilized in agarose-alginate biofilm was twice and thrice the amount reduced by batch culture and cells entrapped in a dialysis sac, respectively.