In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte
In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte
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DOI：
10.1016/j.bbrc.2004.09.086
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发表时间：
2004-11-12
影响因子：
3.1
通讯作者：
Monget, P
中科院分区：
文献类型：
--
作者：
Dadé, S;Callebaut, I;Monget, P
在目前的工作中。我们使用硅减法方法鉴定了6个与NALP5/ MATER相似的小鼠新基因，这些基因的ESTs几乎全部存在于卵子文库中。选择5个基因进行RT-PCR和/或原位杂交。这些实验证实了它们卵母细胞的限制性表达。这些基因中有5个定位在小鼠7号染色体上，如NALP5/MATER，其中3个定位在一个300 kb的簇中。(C) 2004爱思唯尔公司版权所有。
In the present work. we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/ MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER, among them, three are localized in a 300 kb cluster. (C) 2004 Elsevier Inc. All rights reserved.