Elongation and Random Orientation of Bovine Endothelial Cells in Response to Hydrostatic Pressure: Comparison with Response to Shear Stress
Elongation and Random Orientation of Bovine Endothelial Cells in Response to Hydrostatic Pressure: Comparison with Response to Shear Stress
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DOI：
10.1299/jsmec.46.1248
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发表时间：
2003-12
期刊：
影响因子：
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通讯作者：
Y. Sugaya;N. Sakamoto;T. Ohashi;Masaaki Sato
中科院分区：
文献类型：
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作者：
Y. Sugaya;N. Sakamoto;T. Ohashi;Masaaki Sato
研究了暴露于静水压的培养牛内皮细胞（EC）的形态学反应。 ECs在培养基的静水压头下暴露于生理血压24小时。受压的EC表现出显着的伸长和随机方向的取向，以及位于中心的厚应力纤维的发展。与控制条件下不同，加压 EC 还表现出多层结构。暴露于静水压力后，面积和形状指数值显着下降，这与传统流动实验的结果非常吻合。相比之下，新引入的代表细胞形状弯曲度的弯曲度指数对于受压ECs显着增加，而剪切ECs的弯曲度指数与对照没有差异。此外，受压 EC 与无主导方向对齐，而剪切 EC 与流动方向对齐。这些结果表明，内皮细胞可以对施加的机械刺激类型（例如静水压力和流体剪切应力）做出非常具体的反应。
Morphological responses of cultured bovine endothelial cells (ECs) exposed to hydrostatic pressure were investigated. ECs were exposed to physiological blood pressure under a hydrostatic head of culture medium for 24 hours. Pressured ECs exhibited marked elongation and orientation with the random direction, together with development of centrally located, thick stress fibers. Pressured ECs also exhibited multilayered structure unlike under control conditions. The area and the shape index value significantly decreased after exposure to hydrostatic pressure, which were in good agreement with the results from conventional flow-imposed experiments. In contrast, a tortuosity index, which was newly introduced to represent cell shape tortuosity, significantly increased for pressured ECs, while sheared ECs had no difference in turtuosity index from control. In addition, pressured ECs aligned with no predominant direction, while sheared ECs aligned in the flow direction. These results indicate that ECs can respond very specifically to the type of imposed mechanical stimuli such as hydrostatic pressure and fluid shear stress.