Selective nuclear transport of the Drosophila morphogen dorsal can be established by a signaling pathway involving the transmembrane protein Toll and protein kinase A.
Selective nuclear transport of the Drosophila morphogen dorsal can be established by a signaling pathway involving the transmembrane protein Toll and protein kinase A.
复制标题
果蝇形态发生素背侧的选择性核转运可以通过涉及跨膜蛋白 Toll 和蛋白激酶 A 的信号通路建立。
DOI：
10.1101/gad.6.9.1654
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发表时间：
1992
影响因子：
10.5
通讯作者：
Manley,JL
中科院分区：
文献类型：
--
作者：
Norris,JL;Manley,JL
在早期果蝇胚胎中建立背腹极性需要母体形态原背（dl）的浓度梯度。这种浓度梯度是通过dl的选择性核转运建立的，因此dl蛋白仅存在于腹侧核中。dl核定位需要11个基因的活性。这些基因之一，Toll，编码的跨膜蛋白，似乎发挥最直接的作用，在调节dl定位。我们研究了在共转染的Schneider细胞中Toll对dl的影响，以深入了解这些蛋白质之间相互作用的性质。我们已经发现，Toll可以增强dl的核定位，并且独立地增强dl在细胞核中激活转录的能力。我们目前的证据表明，从Toll到DL的信号通路涉及蛋白激酶A（PKA）和核运输和激活DL的结果从磷酸化DL PKA。我们讨论的意义，这些结果与果蝇胚胎发育和哺乳动物转录因子NF-κ B的调节。
Establishment of dorsal-ventral polarity in the early Drosophila embryo requires a concentration gradient of the maternal morphogen dorsal (dl). This concentration gradient is established by selective nuclear transport of dl so that dl protein is present only in ventral nuclei. The activity of 11 genes is required for dl nuclear localization. One of these genes, Toll, encodes a transmembrane protein that appears to play the most direct role in regulating dl localization. We have examined the effects of Toll on dl in cotransfected Schneider cells to gain insight into the nature of the interaction between these proteins. We have found that Toll can enhance the nuclear localization of dl and, independently, the ability of dl to activate transcription once in the nucleus. We present evidence that the signaling pathway from Toll to dl involves protein kinase A (PKA) and that nuclear transport and activation of dl results from phosphorylation of dl by PKA. We discuss the significance of these results with respect both to Drosophila embryogenesis and to the regulation of the mammalian transcription factor NF-kappa B.