Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2
Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2
复制标题
冠状病毒刺突蛋白和宿主 ACE2 受体的组成和差异预测 SARS-CoV-2 的潜在中间宿主
DOI：
10.1002/jmv.25726
复制
发表时间：
2020-06-01
影响因子：
12.7
通讯作者：
Liu, Long
中科院分区：
文献类型：
--
作者：
Liu, Zhixin;Xiao, Xiao;Liu, Long
从2002年初到2012年，严重呼吸综合征冠状病毒（SARS-CoV）和中东呼吸综合征冠状病毒（MERS-CoV）跨越物种屏障感染人类，分别造成数千人感染和数百人死亡。目前，发现了一种新型冠状病毒（SARS-CoV-2），它已成为2019冠状病毒病（COVID-19）爆发的原因。截至2020年2月18日，中国共有72 533例新冠肺炎确诊病例（包括10 644例重症病例），1872例死亡。SARS-CoV-2在公众中传播，由于其人与人之间的传播，造成了巨大的负担。然而，SARS-CoV-2的中间宿主仍不清楚。寻找可能的SARS-CoV-2中间宿主是防止疫情进一步蔓延的当务之急。本研究采用系统比较和分析的方法，预测冠状病毒刺突蛋白受体结合域（receptor-binding domain，RBD）与宿主受体血管紧张素转换酶2（angiotensin-converting enzyme 2，ACE 2）的相互作用。S蛋白RBD的关键氨基酸与ACE 2之间的相互作用表明，除了穿山甲和蛇，如前所述，海龟（刺龟，海龟和中华鳖）可能作为潜在的中间宿主传播SARS-CoV-2给人类。
From the beginning of 2002 and 2012, severe respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) crossed the species barriers to infect humans, causing thousands of infections and hundreds of deaths, respectively. Currently, a novel coronavirus (SARS-CoV-2), which has become the cause of the outbreak of Coronavirus Disease 2019 (COVID-19), was discovered. Until 18 February 2020, there were 72 533 confirmed COVID-19 cases (including 10 644 severe cases) and 1872 deaths in China. SARS-CoV-2 is spreading among the public and causing substantial burden due to its human-to-human transmission. However, the intermediate host of SARS-CoV-2 is still unclear. Finding the possible intermediate host of SARS-CoV-2 is imperative to prevent further spread of the epidemic. In this study, we used systematic comparison and analysis to predict the interaction between the receptor-binding domain (RBD) of coronavirus spike protein and the host receptor, angiotensin-converting enzyme 2 (ACE2). The interaction between the key amino acids of S protein RBD and ACE2 indicated that, other than pangolins and snakes, as previously suggested, turtles (Chrysemys picta bellii, Chelonia mydas, and Pelodiscus sinensis) may act as the potential intermediate hosts transmitting SARS-CoV-2 to humans.