Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity.
Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity.
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EDNA水溶液的年度时间序列分析揭示了湖泊生态系统生物多样性的生态相关动力学。
DOI：
10.1038/ncomms14087
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发表时间：
2017-01-18
影响因子：
16.6
通讯作者：
Creer S
中科院分区：
文献类型：
--
作者：
Bista I;Carvalho GR;Walsh K;Seymour M;Hajibabaei M;Lallias D;Christmas M;Creer S
环境DNA(EDNA)在生物多样性评估中的使用提供了灵敏度、吞吐量以及生态系统多样性和功能的同步测量的阶梯式变化。然而，仍然有必要通过同时测量EDNA和生物群来检验EDNA在野外的持久性。在这里，我们使用两个不同长度的标记的变位编码，来自于水相湖泊Edna的年度时间序列，以检查生态系统生物多样性和具有重要生态意义的大型无脊椎动物(双翅目：摇蚊科)群体的时间变化。这些分析允许随着时间的推移对分类群丰富度和群落组成(β多样性)进行不同程度的检测和验证，较短的EDNA片段主导着EDNA群落。EDNA、群落DNA、分类学和英国物种丰度数据之间的比较进一步表明，通过不同的方法得出的多样性估计之间存在显著的关系。我们的结果揭示了EDNA的时间动态，并验证了EDNA元编码在生态系统尺度上跟踪季节多样性的有效性。可以从包括水在内的环境样本中提取大型生物类群的DNA，并用于评估该地区的生物多样性。在这里，Bista和他的同事们展示了可以通过分析环境DNA(EDNA)来检测湖泊无脊椎动物群落生物多样性的时间变化。
The use of environmental DNA (eDNA) in biodiversity assessments offers a step-change in sensitivity, throughput and simultaneous measures of ecosystem diversity and function. There remains, however, a need to examine eDNA persistence in the wild through simultaneous temporal measures of eDNA and biota. Here, we use metabarcoding of two markers of different lengths, derived from an annual time series of aqueous lake eDNA to examine temporal shifts in ecosystem biodiversity and in an ecologically important group of macroinvertebrates (Diptera: Chironomidae). The analyses allow different levels of detection and validation of taxon richness and community composition (β-diversity) through time, with shorter eDNA fragments dominating the eDNA community. Comparisons between eDNA, community DNA, taxonomy and UK species abundance data further show significant relationships between diversity estimates derived across the disparate methodologies. Our results reveal the temporal dynamics of eDNA and validate the utility of eDNA metabarcoding for tracking seasonal diversity at the ecosystem scale. DNA from macrobial taxa can be extracted from environmental samples, including water, and be used to assess biodiversity in the region. Here, Bista and colleagues show that temporal shifts in the biodiversity of a lake invertebrate community can be detected by analysis of environmental DNA (eDNA).