Measurement of the force-velocity relation for growing microtubules
Measurement of the force-velocity relation for growing microtubules
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DOI：
10.1126/science.278.5339.856
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发表时间：
1997-10-31
期刊：
影响因子：
56.9
通讯作者：
Yurke, B
中科院分区：
文献类型：
--
作者：
Dogterom, M;Yurke, B
由蛋白质聚合产生的力对于各种形式的细胞运动是重要的。例如，组装微管被认为在有丝分裂期间对染色体施加推力。通过将微管的一端连接到基底上并使它们在另一端推压微型刚性屏障来测量单个微管可以产生的力。随后的屈曲的微管进行了分析，以确定每个微管端部上的力和生长速度。生长速度从零力时的1.2微米/分钟降低到3至4皮牛顿力时的0.2微米/分钟。力-速度关系符合衰减指数，与理论模型一致，但衰减速率比预测的要快。
Forces generated by protein polymerization are important for various forms of cellular motility. Assembling microtubules, for instance, are believed to exert pushing forces on chromosomes during mitosis. The force that a single microtubule can generate was measured by attaching microtubules to a substrate at one end and causing them lo push against a microfabricated rigid barrier at the other end. The subsequent buckling of the microtubules was analyzed to determine both the force on each microtubule end and the growth velocity. The growth velocity decreased from 1.2 micrometers per minute at zero force to 0.2 micrometer per minute at forces of 3 to 4 piconewtons. The force-velocity relation fits well to a decaying exponential, in agreement with theoretical models, but the rate of decay is faster than predicted.