RhoA regulates initiation of invagination, but not convergent extension, during sea urchin gastrulation
RhoA regulates initiation of invagination, but not convergent extension, during sea urchin gastrulation
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DOI：
10.1016/j.ydbio.2005.12.031
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发表时间：
2006-04-01
影响因子：
2.7
通讯作者：
McClay, DR
中科院分区：
文献类型：
--
作者：
Beane, WS;Gross, JM;McClay, DR
在原肠胚形成期间，利用细胞形状变化、细胞重排、丝状伪足延伸和会聚延伸运动来拉长和塑造新生肠管，从而形成原肠。尽管通过仔细的形态学检查和分子扰动已经了解了很多信息，但这些事件是如何协调的仍然未知。这项研究报告称，RhoA 对于触发海胆胚胎中的 archenteron 内陷是必需的。抑制 RhoA 会导致无法启动内陷运动，而组成型活性 RhoA 会诱导弓肠细胞过早内陷，并完成通常伴随内陷开始的肌动蛋白重排和细胞外基质分泌。尽管已报道RhoA活性控制脊椎动物胚胎中的会聚伸展运动，但本文的实验表明RhoA活性并不调节海胆原肠胚形成期间的会聚伸展运动。相反，结果支持这样的假设：RhoA 作为启动内陷的触发器，一旦启动发生，RhoA 活性就不再参与随后的原肠胚形成运动。 (c) 2006 Elsevier Inc. 保留所有权利。
During gastrulation, the archenteron is formed using Cell shape changes, cell rearrangements, filopodial extensions, and convergent extension movements to elongate and shape the nascent gut tube. How these events are coordinated remains unknown, although much has been learned from careful morphological examinations and molecular perturbations. This study reports that RhoA is necessary to trigger archenteron invagination in the sea urchin embryo. Inhibition of RhoA results in a failure to initiate invagination movements, while constitutively active RhoA induces precocious invagination of the archenteron, complete with the actin rearrangements and extracellular matrix secretions that normally accompany the onset of invagination. Although RhoA activity has been reported to control convergent extension movements in vertebrate embryos, experiments herein show that RhoA activity does not regulate convergent extension movements during sea urchin gastrulation. Instead, the results support the hypothesis that RhoA serves as a trigger to initiate invagination, and once initiation occurs, RhoA activity is no longer involved in subsequent gastrulation movements. (c) 2006 Elsevier Inc. All rights reserved.