Plant root PET: visualization of photosynthate translocation to roots in rice plant
Plant root PET: visualization of photosynthate translocation to roots in rice plant
复制标题
植物根部 PET：水稻植物光合产物转移至根部的可视化
作者：
Y. Miyoshi;Y. Nagao;M. Yamaguchi;N. Suzui;Y.;N. Kawachi;E. Yoshida;S. Takyu;H. Tashima;T. Yamaya;N. Kuya;S. Teramoto;Y. Uga
根是植物吸收水分和养分所必需的。为了提高作物产量，有必要了解根的发育及其在地下的作用。尤其是光合作用产物从植物叶片向根的转运是影响根伸长、对土壤环境的适应和养分吸收的重要生理功能。用正电子发射断层扫描(PET)和~(11)C示踪剂研究了植物根系的运转动态。然而，由于视差误差，在视场(FOV)外围区域周围形成的根部的空间分辨率会降低。在这项研究中，为了克服这个问题，我们开发了一个应用四层相互作用深度探测器的小型OpenPET原型。我们展示了11C-光合作用产物转运到水稻根部的成像能力，这种能力贯穿于整个PET领域。我们还试图利用高通量X射线计算机层析成像(CT)系统获取同一试验植物的根的结构信息。因此，我们成功地可视化了围绕FOV外围区域发展的根结构，并在不降低空间分辨率的情况下成像了11C-光合作用积累到这些区域的根。从获得的图像中，我们还成功地评估了不同根的易位动力学。高通量CT系统和OpenPET原型的结合使用被证明是适合于牙根的结构和功能分析的。
Roots are essential to plants for uptake of water and nutrients. For the improvement of crop production, it is necessary to understand the elucidation of the root development and its function under the ground. Especially, photosynthate translocation from plant leaves to roots is an important physiological function that affects the root elongation, adaptation to the soil environment and nutrients uptake. To evaluate the translocation dynamics to roots, positron emission tomography (PET) and 11C tracer have been used. However, the spatial resolution is degraded at roots that develop around the peripheral area of field of view (FOV) due to parallax errors. In this study, to overcome this problem, we developed a small OpenPET prototype applying four-layer depth-of-interaction detectors. We demonstrated the imaging capability of 11C-photosynthate translocation to rice roots that develop throughout the entire PET field. We also tried to obtain structural information of roots by high-throughput X-ray computerized tomography (CT) system using the same test plant. As a result, we succeeded in visualizing the root structure that developed around the peripheral region of FOV and imaging the accumulation of 11C-photosynthate to the roots in those areas without degrading the spatial resolution. From obtained images, we also succeeded in evaluating the translocation dynamics varied by roots. The combined use of the high-throughput CT system and the OpenPET prototype was demonstrated to be appropriate for structural and functional analysis of roots.
影响因子：
2.8
作者：
Loening, Andreas Markus;Gambhir, Sanjiv Sam
通讯作者：
Gambhir, Sanjiv Sam
影响因子：
7.4
作者：
Leitner, Daniel;Felderer, Bernd;Schnepf, Andrea
通讯作者：
Schnepf, Andrea