Janthinobacterium violaceinigrum sp. nov., Janthinobacterium aquaticum sp. nov. and Janthinobacterium rivuli sp. nov., isolated from a subtropical stream in China
Janthinobacterium violaceinigrum sp. nov., Janthinobacterium aquaticum sp. nov. and Janthinobacterium rivuli sp. nov., isolated from a subtropical stream in China
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紫色珍蒂诺杆菌 sp.
DOI：
10.1099/ijsem.0.004097
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Xu, Meiying
中科院分区：
文献类型：
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作者：
Lu, Huibin;Deng, Tongchu;Xu, Meiying
从我国亚热带河流中分离到4株菌株，命名为FT 13 W（T）、FT 14 W、FT 58 W（T）和FT 68 W（T）。所有菌株革兰氏染色阴性，过氧化氢酶和氧化酶阳性，杆状，有鞭毛，能运动。基于16 S rRNA基因序列的比较表明，菌株FT 13 W（T）、FT 14 W、FT 58 W（T）和FT 68 W（T）属于Janthinobacterium属，与Janthinobacterium lividum DSM 1522（T）、Janthinobacterium agaricidamnosum DSM 9628（T）和“Janthinobacterium svalbardensis JA-1”的165个rRNA基因的相似性在98.8- 99.7%之间。除菌株FT 13 W（T）与FT 14 W之间的平均核苷酸同一性（ANI）为96.8%外，其余7个菌株基因组间的ANI值在79.0- 92.2%之间。经测定，菌株FT 13 W（T）、FT 14 W、FT 58 W（T）和FT 68 W（T）的呼吸醌含量为0 ~ 8。主要脂肪酸为C-16：1、omega 7 c、C-16（：0）、C-18：1、omega 7 c和C-1（2：）0。极性脂质包括磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。菌株FT 13 W（T）、FT 14 W、FT 58 W（T）和FT 68 W（T）的基因组大小分别为6.45、6.38、5.73和6.37 Mbp，G+C含量分别为63.4、63.7、61.6和63.1 mol%。综合表型、生化、基因型和ANI等数据，菌株FT 13 W（T）和FT 14 W应属于同一种。这四种菌株被认为代表了Janthinobacterium属内的三个新物种，其名称为Janthinobacterium violaceinigrum sp. nov.（模式菌株FT 13 W（T）=GDMCC 1.1638（T）=KACC 21319（T）），水生贾森杆菌（Janthinobacterium aquaticum sp. nov.）（FT 58 W（T）=GDMCC 1.1676（T）=KACC 21468（T））和溪生贾森杆菌（Janthinobacterium rivuli sp. nov.）（FT 68 W（T）=GDMCC 1.1677（T）=KACC 21469（（T）提出。
Four strains assigned the names FT13W(T), FT14W, FT58W(T) and FT68W(T) were isolated from a subtropical stream in PR China. All the strains were Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile with flagella. Comparisons based on 16S rRNA gene sequences showed that strains FT13W(T), FT14W, FT58W(T) and FT68W(T) belonged to genus Janthinobacterium and shared 165 rRNA gene similarities in the range of 98.8-99.7 % with Janthinobacterium lividum DSM 1522(T), Janthinobacterium agaricidamnosum DSM 9628(T) and 'Janthinobacterium svalbardensis JA-1', respectively. The calculated pairwise average nucleotide identity (ANI) values among the genomes of above seven strains were in the range of 79.0-92.2 %, except that the ANI value was 96.8% between strain FT13W(T) and FT14W. The respiratory quinone of strains FT13W(T), FT14W, FT58W(T) and FT68W(T) was determined to be 0-8. The major fatty acids were C-16:1, omega 7c, C-16(:0), C-18:1, omega 7c and C-1(2:)0. The polar lipids included phosphatidy-lethanolamine, phosphatidylglycerol and one unidentified phospholipid. The genome sizes of strains FT13W(T), FT14W, FT58W(T) and FT68W(T) were 6.45, 6.38, 5.73 and 6.37 Mbp with G+C contents of 63.4, 63.7, 61.6 and 63.1 mol%, respectively. Combining phenotypic, biochemical, genotypic and ANI data, strain FT13W(T) and FT14W should belong to the same species. The four strains were considered to represent three novel species within genus Janthinobacterium, for which the names Janthinobacterium violaceinigrum sp. nov. (type strain FT13W(T)=GDMCC 1.1638(T)=KACC 21319(T)), Janthinobacterium aquaticum sp. nov. (FT58W(T)=GDMCC 1.1676(T)=KACC 21468(T) ) and Janthinobacterium rivuli sp. nov. (FT68W(T)=GDMCC 1.1677(T)=KACC 21469(T)) are proposed.