Generating fixed concentration arrays in a microfluidic device
Generating fixed concentration arrays in a microfluidic device
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DOI：
10.1016/s0925-4005(03)00129-1
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发表时间：
2003-07-01
影响因子：
8.4
通讯作者：
Cremer, PS
中科院分区：
文献类型：
--
作者：
Holden, MA;Kumar, S;Cremer, PS
我们设计并建造了一个层流微流体扩散稀释器（muDD），以获得固定的浓度梯度。本研究的驱动力是希望最大限度地减少高通量测量中作为浓度函数所消耗的珍贵分析物的量。这是通过设计一种微流体系统实现的，该系统能够通过非常缓慢的压力驱动流输送微量的分析物。muDD由一个y型接头组成，该接头允许两种不同的流进入主通道，最终分裂成一个独立的微通道线性阵列。阵列技术是基于纳升量的分析物从一个流体流到另一个流体流的对流/扩散传输。muDD设计允许输出通道显示预定的分析物浓度值，可以通过调节流量来控制。实验在500 ~ 50 nl/min的流速范围内进行。对主通道中对流/扩散输运的理论研究已经作为Peclet数和归一化通道尺寸的函数进行了。这些结果通过荧光显微镜实验以及二维和三维数值模拟得到了验证。计算结果与实验结果吻合较好，验证了muDD设计的正确性。(C) 2003 Elsevier Science B.V.版权所有
We have designed and built a laminar microfluidic diffusion diluter (muDD) to obtain fixed concentration gradients inside lithographically patterned lab-on-a-chip architectures. The driving force for this investigation was the desire to minimize the amount of precious analyte consumed in high throughput measurements performed as a function of concentration. This was achieved by engineering a microfluidic system capable of delivering minute volumes of analyte by very slow pressure-driven flow. The muDD consists of a Y-junction that allows inflow of two different streams into a main channel, which eventually splits into a linear array of independent microchannels. The arraying technique is based on convective/diffusive transport of nanoliter quantities of an analyte from one fluid stream into the other. The muDD design allows output channels to exhibit predetermined analyte concentration values, which can be controlled by regulating the flow rate. Experiments were performed for flow rates ranging from 500 to 50 nl/min. Theoretical studies of convective/diffusive transport in the main channel have been performed as a function of the Peclet number and the normalized channel dimensions. These results were validated using fluorescence microscopy experiments as well as two- and three-dimensional numerical simulations. The computational results compared well with the experimental measurements, validating the muDD design. (C) 2003 Elsevier Science B.V. All rights reserved.