Size dependent immune response after subcutaneous, oral and intranasal administration of BSA loaded nanospheres
Size dependent immune response after subcutaneous, oral and intranasal administration of BSA loaded nanospheres
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DOI：
10.1016/s0264-410x(02)00435-8
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发表时间：
2002-11-22
期刊：
影响因子：
5.5
通讯作者：
Pedraz, JL
中科院分区：
文献类型：
--
作者：
Gutierro, I;Hernández, RM;Pedraz, JL
BSA包埋在不同大小的颗粒（200，500和1000 nm）由聚（D，L-乳酸-共-乙醇酸）通过复乳法制备。将颗粒鼻内、口服或皮下给予Balb/c小鼠，并将引发的血清IgG、IgG 1和IgG 2a应答与皮下给予游离抗原、用弗氏完全佐剂（FCA）1：1乳化的游离抗原或用Al（OH）给予的游离抗原所获得的应答进行比较（3）。施用1000 nm颗粒通常引起比施用500或200 nm大小的纳米球获得的血清IgG应答更高的血清IgG应答，500 nm颗粒的免疫应答与通过皮下和口服途径施用200 nm获得的免疫应答相似，并且通过鼻内途径更高。PLGA纳米颗粒可以通过所研究的三种途径引起血清IgG 2a应答。皮下、经口和鼻内给予不同微球后，血清IgG 2a/IgG 1比值无显著差异，但与游离抗原或吸附于明矾的游离抗原给药相比，这些比值通常较高。给药途径会影响游离抗原给药后的血清IgG 2a/IgG 1比，但不会影响颗粒给药后的血清IgG 2a/IgG 1比。因此，不同尺寸颗粒诱导的总血清IgG应答的差异不会导致IgG 1或IgG 2a型免疫应答的差异，表明在PLGA颗粒检测的所有情况下，抗原加工和呈递相似。(C)2002爱思唯尔科技有限公司。保留所有权利。
BSA was entrapped in particles of different sizes (200, 500 and 1000 nm) prepared from poly(D,L-lactic-co-glycolic) acid by a double emulsion method. The particles were given, either intranasally, orally or subcutaneously, to Balb/c mice and the serum IgG, IgG1 and IgG2a response elicited was compared to that obtained by the subcutaneous administration of either free antigen, free antigen emulsified 1:1 with Freund's Complete Adjuvant (FCA), or free antigen administered with Al(OH)(3). The administration of 1000 nm particles generally elicited a higher serum IgG response than that obtained with the administration of 500 or 200 nm sized nanospheres, the immune response for 500 nm particles being similar than that obtained with 200 nm by the subcutaneous and the oral route, and higher by the intranasal route. PLGA nanoparticles can elicit serum IgG2a responses by the three routes studied. No significant differences on the serum IgG2a/IgG1 ratios were found after the subcutaneous, the oral and the intranasal administration of the different spheres but those were in general higher compared to the administration of either free antigen or free antigen adsorbed to alum. The route of administration influences the serum IgG2a/IgG1 ratio after the administration of free antigen, but not after the administration of the particles. Therefore, differences on the total serum IgG response induced by particles of different sizes do not result in differences on the IgG1 or IgG2a-type immune responses, suggesting that the antigen processing and presentation is similar in all cases tested for PLGA particles. (C) 2002 Elsevier Science Ltd. All rights reserved.