Hepatocyte growth factor promotes neuronal differentiation of neural stem cells derived from embryonic stem cells
Hepatocyte growth factor promotes neuronal differentiation of neural stem cells derived from embryonic stem cells
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DOI：
10.1097/00001756-200401190-00002
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发表时间：
2004-01-19
期刊：
影响因子：
1.7
通讯作者：
Sakai, N
中科院分区：
文献类型：
--
作者：
Kato, M;Yoshimura, S;Sakai, N
我们以前报道过肝细胞生长因子（HGF）促进小鼠胚胎脑神经干细胞（NSCs）的神经球增殖和神经元分化。在这项研究中，从小鼠胚胎干（ES）细胞球产生的漂浮培养后，共培养的PA 6基质细胞。与胚胎脑神经球的行为相反，在漂浮培养物的生长培养基中加入HGF减少了ES细胞神经球的数量。当使用MAP-2抗体对球体进行染色时，在与HGF一起培养的球体中观察到更多的MAP-2阳性细胞。当HGF加入到生长和/或分化培养基中时，也获得了更多的MAP-2阳性细胞。这些结果表明，HGF促进ES细胞来源的神经干细胞的神经元分化。
We previously reported that hepatocyte growth factor (HGF) promoted proliferation of neurospheres and neuronal differentiation of neural stem cells (NSCs) derived from mouse embryonic brain. In this study, spheres from mouse embryonic stem (ES) cells were generated by floating culture following co-culture on PA6 stromal cells. In contrast to the behavior of the neurospheres derived from embryonic brain, addition of HGF to the growth medium of the floating cultures decreased the number of spheres derived from ES cells. When spheres were stained using a MAP-2 antibody, more MAP-2-positive cells were observed in spheres cultured with HGF. When HGF was added to the growth and/or differentiation medium, more MAP-2-positive cells were also obtained. These results suggest that HGF promotes neuronal differentiation of NSCs derived from ES cells.