Continuous amperometric detection of co-released serotonin and melatonin from the mucosa in the ileum
Continuous amperometric detection of co-released serotonin and melatonin from the mucosa in the ileum
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DOI：
10.1039/b717034c
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
Patel, Bhavik Anil
中科院分区：
文献类型：
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作者：
Patel, Bhavik Anil
5-羟色胺（5-HT）和褪黑激素（MEL）是众所周知的神经递质和旁分泌信号分子。这两种化合物都存在于胃肠道粘膜中的肠嗜铬（EC）细胞中，并被认为在控制肠道运动中发挥作用。迄今为止，还没有用于检测这两种分子的实时分析方法，并且不清楚MEL是否实际上从EC细胞中释放。本文用掺硼金刚石（BDD）微电极记录兔回肠粘膜EC细胞的5-HT和MEL溢出。的BDD微电极是非常稳定和敏感的测量两种化合物进行评估时，使用差分脉冲伏安法（DPV）和流动注射分析（FIA），使用安培检测。在粘膜中检测到MEL释放，其中MEL最可能来自EC细胞。单个绒毛的机械刺激增加5-HT，但不是MEL溢出。应用5-羟色胺转运体（SERT）抑制剂氟西汀，提高了5-HT，但不是MEL信号。两种胃肠化合物释放量的差异及其释放机制将为EC细胞和疾病状态的生理学提供见解。
Serotonin (5-HT) and melatonin (MEL) are well known neurotransmitters and paracrine signalling molecules. Both compounds are present in enterochromaffin (EC) cells in the mucosa of the gastrointestinal tract and are thought to play a role in controlling gut motility. To date there are no real-time analytical methods for the detection of these two molecules and it is not clear if MEL is actually released from the EC cells. In this paper, I used boron-doped diamond (BDD) microelectrodes to record 5-HT and MEL overflow from EC cells in the mucosa of rabbit ileum. The BDD microelectrode was extremely stable and sensitive for measurements of both compounds when assessed using differential pulse voltammetry (DPV) and flow injection analysis (FIA) using amperometric detection. MEL release was detected in the mucosa, where it is most likely from the EC cells. Mechanical stimulation of individual villi increased 5-HT but not MEL overflow. Application of the serotonin transporter (SERT) inhibitor, fluoxetine, elevated the 5-HT but not the MEL signal. Differences in the amounts of the two gastrointestinal compounds released and the mechanism of which they are released will provide insights to the physiology of the EC cell and disease states.