Dissecting the genetic components of adaptation of Escherichia coli to the mouse gut
Dissecting the genetic components of adaptation of Escherichia coli to the mouse gut
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DOI：
10.1371/journal.pgen.0040002
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Cerf-Bensussan, Nadine
中科院分区：
文献类型：
--
作者：
Giraud, Antoine;Arous, Safia;Cerf-Bensussan, Nadine
虽然多效性适应性突变被认为是进化的核心，但对下游分子效应知之甚少，这些效应允许适应复杂的生态相关环境。在这里，我们发现大肠杆菌MG 1655通过控制100多个基因的EnvZ/OmpR双组分信号转导系统中的单点突变快速适应无菌小鼠的肠道。由调节EnvZ/OmpR活性的突变所赋予的选择性优势是其对鞭毛蛋白表达和渗透性的独立和累加效应的结果。因此，在体内获得的这些结果表明，全球监管机构可能已经发展到协调活动，需要同时进行微调，在适应复杂的环境和突变，在这样的监管机构允许调整的边界时，两个非常不同的环境之间切换的生理适应。
While pleiotropic adaptive mutations are thought to be central for evolution, little is known on the downstream molecular effects allowing adaptation to complex ecologically relevant environments. Here we show that Escherichia coli MG1655 adapts rapidly to the intestine of germ-free mice by single point mutations in EnvZ/OmpR two-component signal transduction system, which controls more than 100 genes. The selective advantage conferred by the mutations that modulate EnvZ/OmpR activities was the result of their independent and additive effects on flagellin expression and permeability. These results obtained in vivo thus suggest that global regulators may have evolved to coordinate activities that need to be fine-tuned simultaneously during adaptation to complex environments and that mutations in such regulators permit adjustment of the boundaries of physiological adaptation when switching between two very distinct environments.