Altered expression of E-cadherin in gastric cancer tissues and carcinomatous fluid.
Altered expression of E-cadherin in gastric cancer tissues and carcinomatous fluid.
复制标题
DOI：
10.1038/bjc.1992.421
复制
发表时间：
1992-12
影响因子：
8.8
通讯作者：
Niitsu, Y
中科院分区：
文献类型：
--
作者：
Matsuura, K;Kawanishi, J;Fujii, S;Imamura, M;Hirano, S;Takeichi, M;Niitsu, Y
应用免疫组化方法对21例不同组织学类型胃癌组织中E-钙粘素的表达进行了研究。几乎所有高分化和中分化腺癌的癌细胞胞间边界的E-钙粘蛋白染色深度与正常胃粘膜相同。然而，这些组织学类型的单个浸润细胞染色较差。在低分化腺癌中，形成簇的癌细胞染色较浅，而那些浸润的单个癌细胞染色更少。在印戒细胞癌中，几乎没有观察到任何染色。在每种组织学类型中，胃壁不同层的染色模式和强度基本相同。同时对胃癌、胰腺癌癌腹水及肺腺癌胸水癌细胞进行免疫荧光染色。11例标本中，10例为阴性，1例为肺腺癌阳性。通过相差显微镜观察，这些癌细胞包括肺腺癌细胞均未形成明显的细胞-细胞接触。细胞聚集试验证实了上述结果。Western blot分析显示肺腺癌癌细胞中E-cadherin的分子量小于完整的E-cadherin。这些结果表明，癌细胞中E-钙粘蛋白的表达降低和/或功能受损，促进其从原发部位释放以自由渗透到组织或流体中。
Expression of E-cadherin in 21 patients with various histological types of gastric carcinomas was studied by immunoperoxidase staining. Intercellular boundaries of almost all cancer cells in well and moderately differentiated adenocarcinomas stained as deeply for E-cadherin as normal gastric mucosa. However, singly infiltrating cells of those histological types were poorly stained. In poorly differentiated adenocarcinomas, cancer cells forming clusters stained lightly and those infiltrating singly stained even less. In signet ring cell carcinomas, hardly any staining was observed. In each histological type, the staining patterns and intensity at different layers of the gastric wall, were essentially the same. Cancer cells from carcinomatous ascites of gastric adenocarcinomas and pancreatic adenocarcinomas, and those from pleural effusion of lung adenocarcinomas were also studied by immunofluorescence staining. Of 11 specimens, ten were negative and only one from a lung adenocarcinomas was positively stained. By phase-contrast microscopic observations, none of these cancer cells including those from the lung adenocarcinomas, formed obvious cell-cell contacts. Cell aggregation assays confirmed the above results. The molecular weight of E-cadherin of cancer cells of lung adenocarcinomas was less than intact E-cadherin as revealed by Western blot analysis. These results suggest that depressed expression and/or impaired function of E-cadherin in cancer cells, facilitates their liberation from primary sites to infiltrate freely into tissue or fluid.