Evidence of alloreactive T suppressor cells in the maintenance phase of spontaneous tolerance after orthotopic liver transplantation in the rat.
Evidence of alloreactive T suppressor cells in the maintenance phase of spontaneous tolerance after orthotopic liver transplantation in the rat.
复制标题
大鼠原位肝移植后自发耐受维持期同种反应性 T 抑制细胞的证据。
作者：
M. Knoop;J. Pratt;I. Hutchinson
在DA->PVG大鼠品系组合中，肝同种异体移植物自发耐受，并诱导对供体抗原的全身耐受状态。使用该品系组合中的30个长期（> 100天）存活的肝受体来研究肝移植后维持期耐受的体液和细胞机制。在腘淋巴结试验中，肝移植或天然PVG大鼠的脾细胞在（DAxPVG）F1杂交大鼠中显示出类似的增殖反应，表明这些细胞的移植物抗宿主反应能力不受影响。使用过继转移试验，长期存活的肝脏受体的T-脾细胞（1 × 10（7）细胞，MRC Ox 19+，MRC Ox 12-，CD 5）能够抑制放射（300 cGy）PVG受体的DA心脏（供体特异性），DA和AO肾移植物的排斥反应。长期存活的肝脏受体的血清能够抑制排斥反应的半同种异体（DAxPVG）F1心脏移植后，双重注射1毫升血清，而完全同种异体DA心脏以及皮肤和胰十二指肠移植物抵抗被动增强。结果证实了在DA->PVG菌株组合中肝移植后自发耐受的发展，并且在过继转移实验中具有抑制能力的T细胞的证明表明T抑制细胞在肝移植后自发耐受的维持阶段中的作用。
In the DA-->PVG rat strain combination liver allografts are spontaneously tolerated and induce a state of systemic tolerance towards donor antigens. Thirty long-term (> 100 days)-surviving liver recipients in this strain combination were used to study humoral and cellular mechanisms of tolerance in the maintenance phase after liver transplantation. In the popliteal lymph node assay splenocytes of liver grafted or native PVG rats displayed a similar proliferative response in (DAxPVG)F1 hybrid rats, indicating an unaffected capacity of these cells to mount graft-versus-host reactions. Using adoptive transfer assays T-splenocytes (1x10(7) cells, MRC Ox 19+, MRC Ox12-, CD 5) of long-term-surviving liver recipients were able to suppress the rejection of DA hearts (donor-specific), of DA and AO kidney grafts in irradiated (300 cGy) PVG recipients. Serum of long-term-surviving liver recipients was able to suppress the rejection of semiallogeneic (DAxPVG)F1 heart grafts after double injection of 1 ml serum, while fully allogeneic DA heart as well as skin and pancreaticoduodenal grafts were resistant to passive enhancement. The results confirm the development of spontaneous tolerance after liver grafting in the DA-->PVG strain combination, and the demonstration of T cells with suppressive capacity in adoptive transfer experiments indicates a role for T suppressor cells in the maintenance phase of spontaneous tolerance after liver grafting.