Identification of Cultivable Bacteria from Tropical Marine Sponges and Their Biotechnological Potentials
Identification of Cultivable Bacteria from Tropical Marine Sponges and Their Biotechnological Potentials
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DOI：
10.21315/tlsr2018.29.2.13
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发表时间：
2018-01-01
影响因子：
1.4
通讯作者：
Bhubalan, Kesaven
中科院分区：
文献类型：
--
作者：
Amelia, Tan Suet May;Amirul, Al-Ashraf Abdullah;Bhubalan, Kesaven
海洋海绵被认为是海洋生物群落中的细菌热点。水生细菌生物活性复合物和次级代谢产物的研究正在进行中。对丁加奴附近南海沃茨不同种类海绵的可培养细菌进行了鉴定。通过16 S rRNA基因克隆和测序完成分子鉴定。14个细菌物种进行了鉴定，并通过构建一个邻居连接树分子进化遗传学分析6的系统发育关系进行了分析。所确定的物种包括四个细菌类别，即厚壁菌门、放线菌门、α-变形菌门和γ-变形菌门，已知这些细菌与海绵有关。根据以往的相关研究，对所鉴定的细菌的潜在生物技术应用进行了比较和综述。此前已报道了14种可培养分离株的生物技术功能，因此强化了与海绵相关的细菌是科学必需化合物的丰富资源。耐冷细菌，耐冷菌，在温暖的热带沃茨的弹性，为未来的研究具有显着的前景。
Marine sponges are acknowledged as bacterial hotspots in the oceanic biome. Aquatic bacteria are being investigated comprehensively for bioactive complexes and secondary metabolites. Cultivable bacteria associated with different species of sea sponges in South China Sea waters adjacent to Bidong Island, Terengganu were identified. Molecular identification was accomplished using 16S rRNA gene cloning and sequencing. Fourteen bacterial species were identified and their phylogenetic relationships were analysed by constructing a neighbour-joining tree with Molecular Evolutionary Genetics Analysis 6. The identified species encompassed four bacterial classes that were Firmicutes, Actinobacteria, Alphaproteobacteria and Gammaproteobacteria known to have been associated with sponges. The potential biotechnological applications of the identified bacteria were compared and reviewed based on relevant past studies. The biotechnological functions of the 14 cultivable isolates have been previously reported, hence reinforcing that bacteria associated with sponges are an abundant resource of scientifically essential compounds. Resilience of psychrotolerant bacteria, Psychrobacter celer, in warm tropical waters holds notable prospects for future research.