Prevention of Porphyromonas gingivalis-induced oral bone loss following immunization with gingipain R1
Prevention of Porphyromonas gingivalis-induced oral bone loss following immunization with gingipain R1
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DOI：
10.1128/iai.69.12.7959-7963.2001
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发表时间：
2001-12-01
影响因子：
3.1
通讯作者：
Genco, CA
中科院分区：
文献类型：
--
作者：
Gibson, FC;Genco, CA
牙龈卟啉单胞菌的精氨酸牙龈蛋白酶RgpA和RgpB是该生物体的已被充分证实的毒力因子。在结构上，RgpA和RgpB具有几乎相同的催化结构域，而RgpA具有额外的血凝素结构域。在这项研究中，我们研究了这些蛋白质的能力，引发对牙龈卟啉单胞菌介导的口腔骨丢失在小鼠口腔挑战模型的保护。用热灭活的牙龈卟啉单胞菌或纯化的RgpA或RgpB皮下免疫的小鼠具有升高的牙龈卟啉单胞菌特异性免疫球蛋白G水平;然而，只有用牙龈卟啉单胞菌全细胞或RgpA免疫的动物才能免受上颌骨骨丢失。这些数据表明，用RgpA免疫刺激血凝素结构域特异性抗体的产生，这有助于预防牙龈卟啉单胞菌介导的牙周病。
The arginine gingipains RgpA and RgpB of Porphyromonas gingivalis are well-documented virulence factors of this organism. Structurally, RgpA and RgpB have nearly identical catalytic domains, while RgpA possesses an additional hemagglutinin domain. In this study, we examined the abilities of these proteins to elicit protection against A gingivalis-mediated oral bone loss In a murine oral challenge model. Mice immunized subcutaneously with heat-killed A gingivalis or purified RgpA or RgpB possessed elevated levels of P. gingivalis-specific Immunoglobulin G; however, only the animals immunized with P. gingivalis whole cells or RgpA were protected from maxillary bone loss. These data suggest that immunization with RgpA stimulates the production of hemagglutinin domain-specific antibodies, which contribute to the prevention of P. gingivalis-mediated periodontal disease.