Evaluation of nanoparticles as endocytic tracers in cellular microbiology.
Evaluation of nanoparticles as endocytic tracers in cellular microbiology.
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DOI：
10.1039/c3nr01550e
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发表时间：
2013-09
期刊：
影响因子：
6.7
通讯作者：
Yuying Zhang;M. Hensel
中科院分区：
文献类型：
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作者：
Yuying Zhang;M. Hensel
病原体与真核宿主细胞相互作用的研究需要引入荧光探针来可视化病原体的内吞作用、细胞内转运或宿主细胞操纵等过程。在这里，三种类型的荧光纳米粒子（NP），即罗丹明标记的聚甲基丙烯酸酯（PMA）的NP，二氧化硅NP和金NP，被用来标记宿主细胞内溶酶体系统和监测操纵的病原体沙门氏菌肠道。使用活细胞成像，我们研究了NPs在细胞摄取、内吞囊泡和溶酶体的标记以及与病原体的相互作用中的性能。我们表明，荧光金和二氧化硅，但不是PMA纳米颗粒适当地标记宿主细胞结构和有效地跟踪重排的宿主内体系统的细胞内沙门氏菌的活动。二氧化硅纳米粒子轻微聚集，位于沙门氏菌诱导的隔室作为孤立的点，而金纳米粒子均匀分布在这样的结构。二氧化硅和金纳米颗粒对宿主细胞或病原体都没有表现出不利影响，并且是感染生物学的通用工具。
The study of pathogen interactions with eukaryotic host cells requires the introduction of fluorescent probes to visualize processes such as endocytosis, intracellular transport or host cell manipulation by the pathogen. Here, three types of fluorescent nanoparticles (NPs), i.e. Rhodamine-labeled polymethacrylate (PMA) NPs, silica NPs and gold NPs, were employed to label the host cellular endolysosomal system and monitor manipulations by the pathogen Salmonella enterica. Using live cell imaging, we investigated the performance of NPs in cellular uptake, labeling of endocytic vesicles and lysosomes, as well as interaction with the pathogen. We show that fluorescent gold and silica, but not PMA NPs appropriately label host cell structures and efficiently track rearrangements of the host endosomal system by the activities of intracellular Salmonella. Silica NPs slightly aggregated and located in Salmonella-induced compartments as isolated dots, while gold NPs distributed uniformly inside such structures. Both silica and gold NPs exhibited no adverse impact on either host cells or pathogens, and are versatile tools for infection biology.