Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
复制标题
DOI：
10.1016/s0014-5793(04)00034-1
复制
发表时间：
2004-02-27
期刊：
影响因子：
3.5
通讯作者：
Fujita, J
中科院分区：
文献类型：
--
作者：
Gotoh, K;Nonoguchi, K;Fujita, J
肝细胞癌（HCC）是世界上第五大常见癌症。我们构建了富含 HCC 中过表达基因的消减 cDNA 文库。鉴定出的 17 个基因包括分子伴侣、Hsp110、Hsp90B 和 Hsp70-1。进一步分析了 Hsp110 家族成员的表达，在 18 个 HCC 中分别发现 12 个和 14 个 HCC 中 Hsp110 和 Apg-2 转录水平升高，但 Apg-1 未升高。免疫组织化学分析表明肿瘤细胞中蛋白质过度表达。在使用荧光素酶的热变性测定中，Apg-2 具有与 Hsp110 类似的伴侣能力，并显示出抗凋亡活性。这些结果表明 Hsp110 家族成员通过其陪伴活动在肝癌发生中发挥重要作用。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70-1. Expression of the Hsp110 family members was further analyzed, and increased transcript levels of Hsp110 and Apg-2, but not Apg-1, were found in 12 and 14, respectively, of 18 HCCs. Immunohistochemical analysis demonstrated the overexpression of the proteins in tumor cells. Apg-2 had chaperone ability similar to Hsp110 in a thermal denaturation assay using luciferase, and showed anti-apoptotic activity. These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.