CLE-CLAVATA1 peptide-receptor signaling module regulates the expansion of plant root systems in a nitrogen-dependent manner
CLE-CLAVATA1 peptide-receptor signaling module regulates the expansion of plant root systems in a nitrogen-dependent manner
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DOI：
10.1073/pnas.1319953111
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发表时间：
2014-02-04
影响因子：
11.1
通讯作者：
Takahashi, Hideki
中科院分区：
文献类型：
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作者：
Araya, Takao;Miyamoto, Mayu;Takahashi, Hideki
根系的形态可塑性对植物的生存至关重要，因为它使植物能够优化其从土壤环境中吸收水分和养分的能力。在这里，我们表明，由氮（N）-响应CLE（CLAVATA 3/ESR相关）肽和CLAVATA 1（CLV 1）富含亮氨酸重复序列受体样激酶组成的信号模块在拟南芥根脉管系统中表达，并在调节氮缺乏条件下根系的扩张中起着至关重要的作用。CLE 1、CLE 3、CLE 4和CLE 7在缺氮胁迫下诱导表达，主要在根周细胞中表达，过表达抑制侧根原基的生长和侧根原基从主根的萌发。相比之下，clv 1突变体在缺氮条件下表现出侧根原基逐渐生长成侧根。通过引入CLV 1启动子驱动的CLV 1：GFP构建体，在根的韧皮部伴随细胞中产生CLV 1：GFP融合蛋白，从而逆转CLV 1表型。CLE 2、CLE 3、CLE 4和CLE 7在CLV 1突变体中的过度积累表明CLE肽信号的幅度受到CLV 1的反馈调节。当CLE 3在野生型植物中在其自身的启动子下过表达时，侧根的长度与CLE 3 mRNA水平的增加呈负相关;然而，CLE 3的这种抑制作用在clv 1突变体背景中被废除。我们的研究结果确定了N-响应CLECLV 1信号模块作为一个重要的机制，限制性地控制扩展的侧根系统在N-缺乏的环境。
Morphological plasticity of root systems is critically important for plant survival because it allows plants to optimize their capacity to take up water and nutrients from the soil environment. Here we show that a signaling module composed of nitrogen (N)-responsive CLE (CLAVATA3/ESR-related) peptides and the CLAVATA1 (CLV1) leucine-rich repeat receptor-like kinase is expressed in the root vasculature in Arabidopsis thaliana and plays a crucial role in regulating the expansion of the root system under N-deficient conditions. CLE1, -3, -4, and -7 were induced by N deficiency in roots, predominantly expressed in root pericycle cells, and their overexpression repressed the growth of lateral root primordia and their emergence from the primary root. In contrast, clv1 mutants showed progressive outgrowth of lateral root primordia into lateral roots under N-deficient conditions. The clv1 phenotype was reverted by introducing a CLV1 promoter-driven CLV1:GFP construct producing CLV1:GFP fusion proteins in phloem companion cells of roots. The overaccumulation of CLE2, -3, -4, and -7 in clv1 mutants suggested the amplitude of the CLE peptide signals being feedback-regulated by CLV1. When CLE3 was overexpressed under its own promoter in wild-type plants, the length of lateral roots was negatively correlated with increasing CLE3 mRNA levels; however, this inhibitory action of CLE3 was abrogated in the clv1 mutant background. Our findings identify the N-responsive CLECLV1 signaling module as an essential mechanism restrictively controlling the expansion of the lateral root system in N-deficient environments.