Effects of Dark Septate Endophytes Strain A024 on Damping-off Biocontrol, Plant Growth and the Rhizosphere Soil Enviroment of Pinus sylvestris var. mongolica Annual Seedlings
Effects of Dark Septate Endophytes Strain A024 on Damping-off Biocontrol, Plant Growth and the Rhizosphere Soil Enviroment of Pinus sylvestris var. mongolica Annual Seedlings
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深色有隔内生菌A024对樟子松立枯病防制、植物生长及根际土壤环境的影响
DOI：
10.3390/plants9070913
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Song, Ruiqing
中科院分区：
文献类型：
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作者：
Deng, Xun;Song, Xiaoshuang;Song, Ruiqing
暗隔膜内生菌在促进植物生长、促进矿物质吸收、生物防治病害、提高植物抗逆性等方面发挥着重要作用。研究了暗隔内生真菌菌株Phialocephala bamuru A024对倒伏病生防效、植物生长发育、根际土壤养分以及一年生白蜡梅幼苗中细菌群落的影响。对沙棘进行了研究。结果表明，接种白叶枯病菌A024后，β-1，3-葡聚糖酶、几丁质酶活性、可溶性蛋白质和脯氨酸含量等生理指标显著降低。在立枯丝核菌胁迫下，蒙古云杉的生长速率显著升高。接种巴姆鲁霉菌A024后，幼苗生物量、土壤养分、根系结构指数以及土壤酶活性均显著高于对照(p<；0.05)。高通量测序的结果表明，侧柏根际土壤中的微生物结构是不同的。接种Bamuru A024菌剂后的沙打旺根际土壤细菌群落结构与土壤主要营养指标呈显著正相关。
Dark septate endophytes (DSEs) exert a vital role in promoting plant growth, improving mineral absorption, biological disease control, and enhancing plant stress resistance. The effects of dark septate endophyte strain, Phialocephala bamuru A024 on damping-off biocontrol, plant development, nutrients within the rhizosphere soil, as well as bacterial communities in the annual seedlings of P. sylvestris var. Mongolica were studied. According to our findings, following P. bamuru A024 inoculation, the damping-off disease morbidity decreased significantly compared with control, some physiological indices such as β-1,3-glucanase, chitinase enzyme activity as well as a soluble protein and proline content in P. sylvestris var. mongolica were elevated under R. solani stress. After inoculation with P. bamuru A024, the biomass in seedlings, nutrients in soil, root structure index, together with activities of soil enzymes were remarkably up-regulated relative to control (p < 0.05). As suggested by the results of high-throughput sequencing, the microbial structure in the rhizosphere soil of the P. sylvestris var. mongolica showed significant differences (p < 0.05) after P. bamuru A024 inoculation compared to control treatment and the rhizosphere soil bacterial community structure after DSE A024 inoculation was positively correlated to the main soil nutrition indices.